Muganda Apoptosis Methods in Toxicology

1. Auflage 2016
ISBN: 978-1-4939-3588-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 244 Seiten, eBook

Reihe: Methods in Pharmacology and Toxicology

This detailed volume provides a single, valuable reference source for methods that definitively identify and accurately quantify apoptosis. The book begins with common methods utilized to detect and quantitate apoptosis, as well as apoptosis signaling pathways in toxicological and other related research. It continues with multi-parametric and phased apoptosis assays for detecting early and late apoptosis or distinguishing apoptosis from necrosis and autophagy. Subsequent chapters focus on recent advances in real time and high-throughput assays that detect and quantitate apoptosis and apoptosis signaling pathways. Final chapters focus on recent developments in preclinical anticancer therapeutics targeting apoptosis. Written for the Methods in Pharmacology and Toxicology series, chapters feature step-by-step descriptions of the methodologies, as well as expert tips and implementation advice. Vital and authoritative, Apoptosis Methods in Toxicology serves novice scientists as well as experts, utilizing a range of instruments from common laboratory equipment to high-end expensive and automated machinery capable of performing real time apoptotic measurements.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Muganda, Perpetua M.

Inhaltsverzeichnis

An Overview of
Apoptosis Methods in Toxicological Research: Recent Updates.- Detection of
Apoptosis: From Bench Side to Clinical Practice.- Determining the Extent of
Toxicant-Induced Apoptosis Using Concurrent Phased Apoptosis Assays.- Measurement
of Apoptosis by Multiparametric Flow Cytometry.- Detecting Apoptosis,
Autophagy, and Necrosis.- A Low-Cost Method for Tracking the Induction of
Apoptosis Using FRET-Based Activity Sensors in Suspension Cells.- FRET-Based
Measurement of Apoptotic Caspase Activities by High-Throughput Screening Flow
Cytometry.- Automated Ratio Imaging Using Nuclear-Targeted Fret
Probe-Expressing Cells for Apoptosis Detection.- Antibody-Based Proteomic
Analysis of Apoptosis Signaling.- Novel Electrochemical Biosensor for Apoptosis
Evaluation.- Microplate-Based Whole Zebrafish Caspase 3/7 Assay for Screening
Small Molecule Compounds.- Targeting Cancer Cell Death with Small-Molecule
Agents for Potential Therapeutics.- Liposomes in Apoptosis Induction and Cancer
Therapy.