Morgan Gene Therapy Protocols

2. Auflage 2002
ISBN: 978-1-59259-141-1
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 69, 520 Seiten, eBook

Reihe: Methods in Molecular Medicine

Efforts in gene therapy have grown dramatically in recent years. Basic research as well as clinical activity have made exciting progress and are beg- ning to offer renewed hope that gene therapy may be able to deliver novel approaches for the treatment of inherited as well as such acquired diseases as cardiovascular disease and cancer. With the sequencing of the human genome complete, we now have a comprehensive catalog of genes that further expands the potential role of gene therapy into such new fields as tissue engineering. Central to gene therapy is the process of gene transfer; thus, advances in the technology of gene transfer are at the heart of this field’s progress. Numerous technologies, based on a variety of methods (e.g., viral-mediated, physical/ chemical), have been developed to achieve gene transfer. Some of the earliest methods, such as recombinant retroviruses, are still widely used, have undergone significant improvements, and have given rise to new vectors based on lentiviruses.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Morgan, Jeffrey R.

Inhaltsverzeichnis

Poly-L-Lysine-Based Gene Delivery Systems.- Targeted Gene Transfer to Liver Using Protein-DNA Complexes.- Receptor-Directed Molecular Conjugates for Gene Transfer.- Gene Transfer into Muscle by Electroporation In Vivo.- Viral Liposomes.- LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery.- Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery.- Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts.- Regulated Expression of Plasmid-Based Gene Therapies.- Photochemical Transfection Light-Induced, Site-Directed Gene Delivery.- Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun.- Preparation of Pseudotyped Retroviral Vector.- Quantitative Measurement of the Concentration of Active Recombinant Retrovirus.- Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System.- Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells.- Genetically Modified Skin Substitutes.- Bioartificial Muscles in Gene Therapy.- Cytokine Gene-Modified Cell-Based Cancer Vaccines.- HIV-Based Vectors.- Packaging Cell System for Lentivirus Vectors Preparation and Use.- Lentiviral Vectors Preparation and Use.- Simian Foamy Virus Vectors.- Recombinant Feline Immunodeficiency Virus Vectors.- Lentivirus Vector Based on Simian Immunodeficiency Virus.- Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus.- Preparation of Helper-Dependent Adenoviral Vectors.- Construction of First-Generation Adenoviral Vectors.- Preparation of Ovine Adenovirus Vectors.- Highly Purified Recombinant Adeno-Associated Virus Vectors.- High-Titer Stocks of Adeno-Associated Virus from Replicating Amplicons and Herpes Vectors.- Herpes Simplex Virus/Adeno-Associated Virus Hybrid Vectors for Gene Transferto Neurons.- Development of Replication-Defective Herpes Simplex Virus Vectors.