Buch, Englisch, Band 69, 520 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 748 g
Buch, Englisch, Band 69, 520 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 748 g
Reihe: Methods in Molecular Medicine
ISBN: 978-0-89603-869-1
Verlag: Humana Press
In this fully updated and revised 2nd edition of Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. These methods range from those in which new molecular conjugates show great promise for targeting targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven the step-by-step laboratory procedures that enable its successful therapeutic application.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Poly-l-Lysine-Based Gene Delivery Systems: Synthesis, Purification, and Application
Charles P. Lollo, Mariusz G. Banaszczyk, Patricia M. Mullen, Christopher C. Coffin, Dongpei Wu, Alison T. Carlo, Donna L. Bassett, Erin K. Gouveia, and Dennis J. Carlo
Targeted Gene Transfer to Liver Using Protein-DNA Complexes
Catherine H. Wu, Cherie M. Walton, and George Y. Wu
Receptor-Directed Molecular Conjugates for Gene Transfer
Assem G. Ziady and Pamela B. Davis
Gene Transfer into Muscle by Electroporation in Vivo
Jun-ichi Miyazaki and Hiroyuki Aihara
Viral Liposomes: Preparation and Use
Yasufumi Kaneda
LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery
Yadi Tan, Mark Whitmore, Song Li, Peter Frederik, and Leaf Huang
Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery: Preparation and Use
Klaus Schughart and Ulla B. Rasmussen
Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts
Olga A. Igoucheva and Kyonggeun Yoon
Regulated Expression of Plasmid-Based Gene Therapies
Ronald V. Abruzzese, Fiona C. MacLaughlin, Louis C. Smith, and Jeffrey L. Nordstrom
Photochemical Transfection: Light-Induced, Site-Directed Gene Delivery
Lina Prasmickaite, Anders Høgset, and Kristian Berg
Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun
Chia-Feng Kuo, Jeng Hwan Wang, and Ning-Sun Yang
Preparation of Pseudotyped Retroviral Vector
Hong Yu
Quantitative Measurement of the Concentration of Active Recombinant Retrovirus
Stelios T. Andreadis and Jeffrey R. Morgan
Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System: Production and Use
Jarmo Wahlfors and Richard A. Morgan
Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells
William P. Swaney,Enrico M. Novelli, Alfred B. Bahnson, and John A. Barranger
Genetically Modified Skin Substitutes: Preparation and Use
Karen E. Hamoen, Gulsun Erdag, Jennifer L. Cusick, Hinne A. Rakhorst, and Jeffrey R. Morgan
Bioartificial Muscles in Gene Therapy
Courtney Powell, Janet Shansky, Michael Del Tatto, and Herman H. Vandenburgh
Cytokine Gene-Modified Cell-Based Cancer Vaccines
R. Todd Reilly, Jean-Pascal H. Machiels, Leisha A. Emens, and Elizabeth M. Jaffee
HIV-Based Vectors: Preparation and Use
Antonia Follenzi and Luigi Naldini
Packaging Cell System for Lentivirus Vectors: Preparation and Use
Narasimhachar Srinivasakumar
Lentiviral Vectors: Preparation and Use
Lung-Ji Chang and Anne-Kathrin Zaiss
Simian Foamy Virus Vectors: Preparation and Use
Jeonghae Park and Ayalew Mergia
Recombinant Feline Immunodeficiency Virus Vectors: Preparation and Use
Michael A. Curran a
