McEwan, PhD The Nuclear Receptor Superfamily

Methods and Protocols
2. Auflage 2016
ISBN: 978-1-4939-3724-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1443, 250 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume aims to describe a complementary range of molecular, cell biological, and in vivo protocols used to investigate the structure–function of nuclear receptors, together with experimental approaches that may lead to new drugs to selectively target nuclear receptor-associated diseases. The Nuclear Receptor Superfamily, Second Edition will benefit those starting out in the nuclear receptor research field as well as to more established researchers who wish to apply different methods to a particular receptor or research problem. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Nuclear Receptor Superfamily, Second Edition aims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

McEwan, PhD, Iain J.

Mcewan

Inhaltsverzeichnis

The Nuclear Receptor Superfamily- At
Thirty.- Lipid Homeostasis and Ligands for
Liver X Receptors: Identification and Characterization.- Use
of Differential Scanning Fluorimetry to Identify Nuclear Receptor Ligands.- Drug-Discovery Pipeline for Novel
Inhibitors of the Androgen Receptor.- Use
of BRET to Study Protein-Protein Interactions in vitro and in vivo.- Studying
Steroid Receptor Complexes in the Cellular Environment.- Post-translational
Modifications of Steroid Receptors: Phosphorylation.- Mapping protein-DNA Interactions using ChIP-exo and Illumina-based Sequencing.- Methods to Identify
Chromatin Bound Protein Complexes: From Genome-Wide to Locus Specific Approaches.- Measuring the Expression of microRNAs Regulated by
Androgens.- Methods for Identifying and
Quantifying Mrna Expression of Androgen Receptor Splicing Variants in Prostate
Cancer.- Harvesting
Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells.- In
vivo Imaging of Nuclear Receptor Transcriptional Activity.- Development
and Characterisation of Cell-Specific Androgen Receptor Knockout Mice.