Buch, Englisch, 306 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 799 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, 306 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 799 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-4577-2
Verlag: Humana
This volume provides a collection of novel and emerging methods for the generation and application of peptide libraries. Chapter focus on methods and techniques highlighting new avenues for library screening. Written in the Methods in Molecular Biology series format, chapters outline strategies and overview their area or describe specific applications of the method including an introduction, the necessary materials, step-by-step, readily reproducible laboratory and computational protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, aims to be comprehensive guide for researchers in the field.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
My Perspective on OBOC Combinatorial Technology.- Encoded Peptide Library Synthesis and Applications, a Mini-Review.- Resemblance-Ranking Peptide Library as a Representation of a Complex Proteome.- ß-Hairpin Peptidomimetics for Protein-Protein Interaction Inhibition.- Preparation of µSPOT Lactam-Cyclized Peptides for Ligand Optimization.- Construction of Cyclic Peptide-Libraries Immobilized on Gel-type Supports for Screening towards Discovery of Interacting Peptides.- Large Macrocyclic Libraries via Thioether Cyclization.- Protease Specificity Profiling using Synthetic Combinatorial Peptide Libraries and Mass Spectrometry.- Parallel and Automated PNA Synthesis in µSPOT Format.- Late-Stage Functionalization of Peptides on Solid-Phase via Photochemical Decarboxylative Arylation.- Building Blocks for the Screening of Histone Deacetylase Inhibitors using µSPOT.- Parallel Synthesis of Glycopeptide Libraries using Glyco-SPOT Synthesis.- Facile High-Throughput Pre-Purification of Biological Samples for Library-Scale Peptide Interaction Studies.- Fmoc Solid-Phase FIT-PNA Synthesis – Manual and Automated Methodologies.- Guidelines for High-throughput Engineering of Non-Ribosomal Peptide Synthetase Libraries.- RIPP Enzymes for Biosynthetically Derived Cyclic Peptide Libraries.- Quantitative Affinity Screening of Macrocyclic Peptide Libraries using Yeast Surface Display Technology.- Ultra-High Throughput Screening of Cystine-Rich Peptide Libraries via Yeast Surface Display.- The Construction of dsDNA-Based AND-Gate (DBAG) Peptide Library in Mammalian Cells.
