Mandal / Paul Automation and Basic Techniques in Medical Microbiology

1. Auflage 2022
ISBN: 978-1-0716-2372-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 204 Seiten, eBook

This book discusses principles, methodology, and applications of microbiological laboratory techniques . It lays special emphasis on the use of various automated machines that are essential for medical microbiology and diagnostic labs. The book contains eleven major chapters. The first chapter describes the good lab practices which should be followed by the students in all biological, chemistry or microbiology laboratories. The next chapter describes manual and automated characterization of antibiotic resistant microbes, followed by a chapter on genomics based tools and techniques that are integral to research. Further chapters deal with other important techniques like immunology based techniques, spectrophotometry and its various types, MALDI-TOFF and microarrays, each with illustrations and detailed description of the protocols and applications. The book also gives certain important guidelines to the students about the planning the experiment and interpreting results. The book is highly informative and provides latest techniques. It is a handy compendium for graduate and post graduate students, as well as more advanced researchers.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Mandal, Santi M.

Paul, Debarati

Inhaltsverzeichnis

Chapter 1: Good laboratory Practices.- Chapter 2: Automation in medical microbiology.- Chapter 3: Manual and automated characterization of Multi-antibiotic-resistant (MAR) bacteria.- Chapter 4: Rapid microbial genome sequencing techniques and applications.- Chapter 5: SPECTROSCOPY - Principle, Types and Microbiological Applications.- Chapter 6: MALDI- TOF for bacterial identification.- Chapter 7: Enzyme-Linked Immunosorbent Assay (ELISA).- Chapter 8: Isolation of normal microbiota from human body and microbial identification.- Chapter 9: Microarrays and its application in medical microbiology.- Chapter 10: Immunotechnology.- Chapter 11: Advances in Microscopy.

Über Autor(innen)

Dr. Santi M. Mandal obtained his PhD in the field of molecular microbiology and continuing research with major focus in antimicrobial chemotherapy. He visited UTMB-USA and NUS-Singapore for his postdoctoral training. He worked as an Assistant Professor of Microbiology at Vidyasagar University, India. He has published more than 110 research papers in reputed journals and conferred upon several prestigious awards for his research contribution. Currently, he is engaged at Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur, India. Debarati Paul (PhD), currently Associate Professor in Amity Institute of Biotechnology, Amity University, has completed her PhD from the Institute of Microbial Technology-CSIR (Chandigarh) and post doc from EAWAG (Switzerland) and Mississippi State University (USA) for about 5 years, before joining Amity University, UP as Assistant Professor. Her main area of research is genomics, environmental biotechnology, antimicrobial resistance and biodegradation. She has published over 70 papers including research articles, reviews, conference proceedings and posters and book chapters. She has received the prestigious Indo US Science and Technology Forum award and DST travel grants, etc. She also trains students for skill development in Quality Control Chemist Microbiology, since 2019.