E-Book, Englisch, Band 307, 336 Seiten, eBook
Reihe: Methods in Molecular Biology
Lugnier Phosphodiesterase Methods and Protocols
Erscheinungsjahr 2008
ISBN: 978-1-59259-839-7
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark
E-Book, Englisch, Band 307, 336 Seiten, eBook
Reihe: Methods in Molecular Biology
ISBN: 978-1-59259-839-7
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark
Zielgruppe
Research
Autoren/Hrsg.
Weitere Infos & Material
Study of Cyclic Adenosine Monophosphate Microdomains in Cells.- High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains.- Cygnets.- High-Throughput Screening of Phosphodiesterase Activity in Living Cells.- Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts.- Localization of the Cyclic Guanosine 3?,5?-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization.- Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells.- Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B.- Identification of Promoter Elements in the 5?-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene.- Purification of PDE6 Isozymes From Mammalian Retina.- Cyclic Guanosine 5?-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase.- Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies.- Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation.- Crystallization of Cyclic Nucleotide Phosphodiesterases.- Generation of PDE4 Knockout Mice by Gene Targeting.- Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase.- Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases.- Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11.- Analysis of Dimerization Determinants of PDE6 Catalytic Subunits.- Interactions Between Catalytic and Inhibitory Subunits of PDE6.- Purification,Reconstitution on Lipid Vesicles, and Assays of PDE6 and Its Activator G Protein, Transducin.
