Chemical Cytometry

Ultrasensitive Analysis of Single Cells
1. Auflage 2010
ISBN: 978-3-527-32495-8
Verlag: Wiley VCH Verlag GmbH

Buch, Englisch, 247 Seiten, Format (B × H): 176 mm x 250 mm, Gewicht: 642 g

Tying together technological and conceptual advances of the past decade, this seminal book introduces the novel approach of chemical cytometry -- the biochemical analysis of cell constituents at the single cell level. As such, it combines recently developed methods for the handling of single cells, the separation of their components, and the detection and analysis of biomolecular species with application examples from cell biology, neurobiology and molecular medicine.
The editor has succeeded in acquiring contributions from the recognized leaders in the field, resulting in a complete and authoritative overview for both developers as well as users of these new methods.

Autoren/Hrsg.

Lu, Chang

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Medizinische Biotechnologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Chemische Verfahrenstechnik
• Naturwissenschaften Chemie Organische Chemie Biochemie

Inhaltsverzeichnis

Preface
ORIGIN, CURRENT STATUS, AND FUTURE PERSPECTIVES OF CHEMICAL CYTOMETRY
The Cell and Cytometry
Flow and Image Cytometry
Chemical Cytometry
Chemical Cytometry of DNA and mRNA
Metabolic Cytometry
Future Perspectives on Instrumentation for Chemical Cytometry
METABOLIC CYTOMETRY - THE STUDY OF GLYCOSPHINGOLIPID METABOLISM IN SINGLE PRIMARY CELLS OF THE DORSAL ROOT GANGLIA
Introduction
Material and Methods
Results and Discussion
Conclusions
CELL SIGNALING STUDIES AT THE SINGLE-CELL LEVEL
Introduction
Analytes Examined and Reporters Used
Cell Preparation and Reporter Loading
Cell Lysis and Sampling Techniques
Electrophoresis Separation Conditions
Detection
Automation and Throughput
ULTRASENSITIVE DETECTION OF LOW-COPY-NUMBER MOLECULES FROM SINGLE CELLS
Introduction
Microchip Designs for Single-Cell Analysis and/or Cell Manipulation
Ultrasensitive Detection Methods for Single-Cell Analysis
Single-Cell Analysis with Single-Molecule Sensitivity on Integrated Microfluidic Chip
Conclusions
CAPILLARY ELECTROPHORESIS OF NUCLEIC ACIDS AT THE SINGLE-CELL LEVEL
Introduction
On-Line Cell Analysis
Direct Gene and Gene Expression Analysis Without Amplification
Potential Alternative Techniques for Single-Cell Gene and Gene Expression Analysis
Conclusions
MICROFLUIDIC TECHNOLOGY FOR SINGLE-CELL ANALYSIS
Introduction
Biological Significance of Single-Cell Analysis
Microfluidic Devices in Our Laboratories
Materials, Methods, and Protocols
Conclusions
ON-CHIP ELECTROPORATION AND ELECTROFUSION FOR SINGLE-CELL ENGINEERING
Introduction
Single-Cell Electroporation in Microfluidic Devices
Single-Cell Electrofusion in Microfluidic Devices
Conclusions
ELECTROPORATIVE FLOW CYTOMETRY FOR SINGLE-CELL ANALYSIS
Introduction
Flow-Through Electroporation under Constant Voltage
Electroporative Flow Cytometry for Detecting Protein Translocation
Electroporative Flow Cytometry for Measuring Single-Cell Biomechanics
Electroporative Flow Cytometry for Selectively Releasing and Analyzing Specific Intracellular Molecules
Conclusion
ULTRASENSITIVE ANALYSIS OF INDIVIDUAL CELLS VIA DROPLET MICROFLUIDICS
Introduction
Droplet Properties
Droplet Generation
Cell Encapsulation
Droplet Manipulation
Droplet Concentration Control
Temperature Control of Droplets
Detection in Droplets
Conclusions
PROBING EXOCYTOSIS AT SINGLE CELLS USING ELECTROCHEMISTRY
Introduction
Measurement Requirements
Electrode Fabrication
Measurements at Single Cells
Fusion Pore Dynamics
Conclusions
ELECTROCHEMICAL DETERMINATION OF ENZYME ACTIVITY IN SINGLE CELLS
Introduction
Electrochemical Detection Coupled with Capillary Electrophoresis
Voltammetry
Scanning Electrochemical Microscopy (SECM)
High-Throughput ECD
Perspective
SINGLE-CELL MASS SPECTROMETRY
Introduction
Mass Spectrometry
Overall Outlook for Single-Cell MS
OPTICAL SENSING ARRAYS FOR SINGLE-CELL ANALYSIS
Introduction to Fiber-Optic Single-Cell Arrays
Advantages of Fiber-Optic Single-Cell Arrays
Fiber-Optic Arrays
Singe-Cell Arrays for Bacteria
Single-Cell Arrays for Yeast
Single-Cell Arrays for Mammalian Cells
Image and Data Analysis for Single-Cell Arrays
Summary

Über Autor(innen)

Lu, Chang
Chang Lu is an associate professor of chemical engineering at Virginia Tech, Blacksburg (USA). From 2004 to 2009 he was an assistant and associate professor of biological engineering at Purdue University at West Lafayette. He obtained his academic degrees from Peking University and from the University of Illinois at Urbana-Champaign, and received post-doctoral training at Cornell University. His research has been focused on Microfluidic devices for single cell analysis and tissue engineering. Professor Lu has received a number of awards, including the NSF Career Award and the Wallace H. Coulter foundation Early Career Award in Biomedical Engineering.