Lojda / Gossrau / Schiebler Enzyme Histochemistry

A Laboratory Manual
1979
ISBN: 978-3-642-67234-7
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 344 Seiten, eBook

During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

Zielgruppe

Research

Autoren/Hrsg.

Lojda, Z.

Gossrau, R.

Schiebler, T.H.

Inhaltsverzeichnis

A. Introduction.- B. General Considerations.- I. Conditions for the Histochemical Demonstration of Enzymes.- II. Terminology and Classification of Enzymes.- III. Principles of Reactions in Histochemical Methods for the Detection of Enzymes.- 1. Precipitation Reactions.- a. Precipitation Reactions with Metallic Cations.- b. Simultaneous Azo-Coupling.- c. Indigogenic Methods.- d. Tetrazolium Methods.- 2. Successive Azo-Coupling.- 3. Synthesizing Reactions.- 4. Substrate Film Procedures.- IV. Artifacts and Control Reactions.- V. Correlation of Histochemical and Biochemical Findings.- C. Preparation of Tissues.- I. Tissue Sampling.- II. Further Treatment.- 1. Fresh Tissue.- a. Freezing.- b. Preparations of Sections.- c. Freeze-drying of Cryostat Sections.- d. Freeze-Substitution.- 2. Fixation.- 3. Further Treatment of Fixed Tissue.- a. Washing.- b. Freezing and Preparation of Sections.- c. Embedding.- 4. Summary of Pretreatment of Tissue Specimens.- III. Incubation.- D. Detection Methods.- I. Instructions.- II. Hydrolases.- 1. Phosphatases.- a. Alkaline Phosphatase.- b. Acid Phosphatase.- c. 5?-Nucleotidase.- d. Glucose-6-Phosphatase.- e. Adenosine Triphosphatase.- f. Thiamine Pyrophosphatase.- g. Nucleoside Diphosphatase.- 2. Carboxylester-Hydrolases.- a. Nonspecific Esterases.- b. Lipase.- c. Cholinesterases.- 3. Glycosidases.- a. Acid ?-Glucosidase.- b. ?-Galactosidase.- c. Acid ß-Galactosidase.- d. ?-Mannosidase.- e. ß-N-Acetylglucosaminidase.- f. ß-GIucuronidase.- g. Disaccharidases.- ?. Lactase.- ß. Maltase.- ?. Sucrase.- ?. Trehalase.- 4. Peptidases.- a. Aminopeptidase M.- b. Dipeptidyl(amino)peptidase IV.- c. Dipeptidyl(amino)peptidase II.- d. Enteropeptidase.- e. ?-Glutamyltransferase.- 5. Sulfatases.- III. Transferases.- 1. Glycogen Phosphorylase.- 2. Glycogen (Starch) Synthase.- IV. Lyases.- 1. Fructose-Bisphosphate Aldolase.- 2. Carbonate Dehydratase.- V. Oxidoreductases.- 1. Oxidases.- a. Cytochrome c Oxidase.- b. Monophenol Monooxygenase.- c. Amine Oxidase.- d. D-Amino Acid Oxidase and Lactate-2-Monooxygenase.- e. Peroxidase.- 2. Dehydrogenases.- A. Coenzyme-lndependent Dehydrogenases.- a. Succinate Dehydrogenase.- b. Glycerol-3-Phosphate Dehydrogenase.- B. Tetrazolium Reductases.- C. Coenzyme-Dependent Dehydrogenases.- a. 3-Hydroxybutyrate Dehydrogenase.- b. UDPG Dehydrogenase.- c. Hydroxysteroid Dehydrogenases.- d. Glycerol-3-Phosphate Dehydrogenase (NAD+).- e. Glyceraldehydephosphate Dehydrogenase.- f. Glutamate Dehydrogenase.- g. Isoeitrate Dehydrogenase.- h. Glucose-6-Phosphate Dehydrogenase.- i. Phosphogluconate Dehydrogenase.- k. Lactate Dehydrogenase.- I. Malate Dehydrogenase.- m. Malate Dehydrogenase (NADP+).- E. Solutions and Buffers.- F. List of Firms.- G. References.- H. Subject Index and Source List.