Buch, Englisch, Band 336, 200 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 315 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 336, 200 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 315 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61737-511-8
Verlag: Humana Press
In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Fachgebiete
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Labormedizin
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
- Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
Weitere Infos & Material
to the Polymerase Chain Reaction.- Setting Up a Polymerase Chain Reaction Laboratory.- Real-Time Polymerase Chain Reaction and Melting Curve Analysis.- Qualitative and Quantitative Polymerase Chain Reaction-Based Methods for DNA Methylation Analyses.- In-Cell Polymerase Chain Reaction.- Qualitative and Quantitative DNA and RNA Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.- Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatography.- Use of Real-Time Polymerase Chain Reaction for the Detection of Fetal Aneuploidies.- Noninvasive Prenatal Diagnosis by Analysis of Fetal DNA in Maternal Plasma.- Clinical Applications of Plasma Epstein-Barr Virus DNA Analysis and Protocols for the Quantitative Analysis of the Size of Circulating Epstein-Barr Virus DNA.- Molecular Analysis of Circulating RNA in Plasma.- Molecular Analysis of Mitochondrial DNA Point Mutations by Polymerase Chain Reaction.- Novel Applications of Polymerase Chain Reaction to Urinary Nucleic Acid Analysis.- Detection and Quantitation of Circulating Plasmodium falciparum DNA by Polymerase Chain Reaction.- Molecular Diagnosis of Severe Acute Respiratory Syndrome.- Genomic Sequencing of the Severe Acute Respiratory Syndrome-Coronavirus.
