Laser Capture in Microscopy and Microdissection

1. Auflage 2002
ISBN: 978-0-08-049701-3
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 356, 775 Seiten, Web PDF

Reihe: Methods in Enzymology

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. - Basic Principles, Specialized Uses, and Genetic Applications - LCM and its application in genomics and proteomics - Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections - Noncontact laser catapulting for the functional genomics and proteomics - Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more

Autoren/Hrsg.

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 356;10
4;Preface;14
5;Volumes in Series;16
6;Section I: Basic Principles;38
6.1;Chapter 1. Comparison of Current Equipment;40
6.2;Chapter 2. Laser Capture Microdissection and Its Applications in Genomics and Proteomics;49
6.3;Chapter 3. Going in Vivo with Laser Microdissection;62
6.4;Chapter 4. Use of Laser Capture Microdissection to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis;70
6.5;Chapter 5. Optimized Tissue Processing and Staining for Laser Capture Microdissection and Nucleic Acid Retrieval;86
6.6;Chapter 6. Fluorescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections;100
6.7;Chapter 7. Immunoblotting of Single Cell Types Isolated from Frozen Sections by Laser Microdissection;107
6.8;Chapter 8. Noncontact Laser Catapulting: A Basic Procedure for Functional Genomics and Proteomics;117
6.9;Chapter 9. Internal Standards for Laser Microdissection;136
6.10;Chapter 10. Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues;151
7;Section II: Specialized Uses;164
7.1;Chapter 11. Use of Laser Capture Microdissection for Clonal Analysis;166
7.2;Chapter 12. Laser Capture Microdissection in Carcinoma Analysis;173
7.3;Chapter 13. Laser Capture Microdissection to Assess Development;181
7.4;Chapter 14. Application of Laser Capture Microdissection to Proteomics;193
7.5;Chapter 15. Laser Capture Microdissection of Mouse Intestine: Characterizingm mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified Cell Populations;203
7.6;Chapter 16. Laser Capture Microdissection in Pathology;232
7.7;Chapter 17. Use of Laser Capture Microscopy in the Analysis of Mouse Models of Human Diseases;243
7.8;Chapter 18. Use of Laser Microdissection in Complex Tissue;252
7.9;Chapter 19. Assessment of Clonal Relationships in Malignant Lymphomas;260
7.10;Chapter 20. Comparison of Normal and Tumor Cells by Laser Capture Microdissection;276
7.11;Chapter 21. Analysis of Folliculostellate Cells by Laser Capture Microdissection and Reverse Transcription–Polymerase Chain Reaction (LCMRT/ PCR);284
8;Section III: Genetic Applications;293
8.1;Chapter 22. Analysis of Gene Expression;295
8.2;Chapter 23. Analysis of Specific Gene Expression;307
8.3;Chapter 24. Gene Discovery with Laser Capture Microscopy;318
8.4;Chapter 25. DNA Fingerprinting from Cells Captured by Laser Microdissection;325
8.5;Chapter 26. Single Cell PCR in Laser Capture Microscopy;331
8.6;Chapter 27. Assessment of Genetic Heterogeneity in Tumors Using Laser Capture Microdissection;338
8.7;Chapter 28. Gene Mutations: Analysis in Proliferative Prostatic Diseases Using Laser Capture Microdissection;345
8.8;Chapter 29. Use of Laser Capture Microdissection-Generated Targets for Hybridization of High-Density Oligonucleotide Arrays;359
8.9;Chapter 30. Single Cell Gene Mutation Analysis Using Laser- Assisted Microdissection of Tissue Sections;370
8.10;Chapter 31. Methylation in Gene Promoters: Assessment after Laser Capture Microdissection;379
9;Author Index;390
10;Subject Index;412