Kroemer / Tang Ferroptosis

Methods and Protocols
Erscheinungsjahr 2023
ISBN: 978-1-0716-3433-2
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2712, 254 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume provides a comprehensive collection of experimental protocols for investigating ferroptosis in different systems, including cultured cells, animal models, and human tissues. The techniques covered in this book look at various aspects of ferroptosis ranging from the detection of lipid peroxidation to the measurement of glutathione peroxidase activity and the evaluation of mitochondrial morphology. Chapters also discuss basic molecular biology methods such as quantitative PCR and immunoblotting, and advanced imaging techniques such as transmission electron microscopy and confocal fluorescence microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Ferroptosis: Methods and Protocols is a valuable resource for researchers who are interested in studying ferroptosis in different contexts including basic research, drug discovery, and clinical applications.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Kroemer, Guido

Tang, Daolin

Inhaltsverzeichnis

Fluorogenic Probes for Intracellular Iron Detection.- Stratifying Ferroptosis Sensitivity in Cells and Tissues with PALP.- ChIP and ChIRP Assays in Ferroptosis.- PAR-CLIP Assay in Ferroptosis.- Organoids Models of Pancreatic Duct Adenocarcinoma.- Probing Lipid Peroxidation in Ferroptosis: Emphasizing the Utilization of C11-BODIPY-Based Protocols.- Membrane Integrity Assay in Ferroptosis.- LC-MS-Based Redox Phosphoipidomics Analysis in Ferroptosis.- Monitoring Lysosome Function in Ferroptosis.- Monitoring Mitochondria Function in Ferroptosis.- Generation of Organoids and Analysis of Ferroptosis in Organoids.- Analysis of Protein Degradation in Ferroptosis.- Lipidomics Analysis in Ferroptosis.- In-Cell Western Assay in Ferroptosis.- Flow Cytometric Analysis of Regulated Cell Death.- Thermal Shift Assay in Ferroptosis.- Detection of Ferroptosis in Patient-Derived Tumor Models.- Assessment of Ferroptosis in Hematopoietic Stem and Progenitor Cells.- Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence.- Analysis of MicroRNAs in Ferroptosis.- Detection of Ferroptosis in Models of Brain Diseases.