Proteomics

Introduction to Methods and Applications
1. Auflage 2008
ISBN: 978-0-470-05535-9
Verlag: Wiley

Buch, Englisch, 360 Seiten, Format (B × H): 158 mm x 241 mm, Gewicht: 684 g

Introduction to Proteomics is written by seasoned researchers with years of practical experience. In addition to comprehensive discussions of the basic concepts, techniques, and applications of the subject, the text also includes an extensive glossary and a chapter containing laboratory exercises and protocols. While mass spectrometry is central to proteomics, the book discusses all of the analytical techniques a student is likely to need when faced with real-world problems, such as sample preparation, chromatographic and electrophoretic separation, micro-total analysis systems, and bioinformatics.

Autoren/Hrsg.

Kraj, Agnieszka

Silberring, Jerzy

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Contributors xi

Preface xv

1 Introduction to Proteomics and Strategy for Protein Identification 1
Jerzy Silberring

2 Sample Preparation 7
Agnieszka Kraj and Jerzy Silberring

3 Chromatographic Separation Methods 13

3.1 Liquid Chromatography Linked to Mass Spectrometry 15
Piotr Suder

3.2 Multidimensional Liquid Chromatography Combined with Mass Spectrometry 23
Anna Drabik

4 Electrophoretic Separation Methods 31

4.1 One-Dimensional Gel Electrophoresis 33
Anna Drabik and Piotr Laidler

4.2 Two-Dimensional Gel Electrophoresis 43
Anna Bierczynska-Krzysik and Gert Lubec

5 Capillary Electrophoresis 55
Marek Noga

6 Protein Blotting 65
Ibeth Guevara-Lora and Andrzej Kozik

7 Introduction to Protein and Peptide Mass Spectrometry 77

7.1 Electrospray Ionization 79
Piotr Suder

7.2 Matrix-Assisted Laser Desorption/Ionization 89
Agnieszka Kraj and Marcus Macht

7.3 Imaging Mass Spectrometry 101
Malgorzata Iwona Szynkowska and Jerzy Silberring

7.4 Fragmentation and Sequencing in Proteomics 113
Piotr Suder

7.5 Protein Identification by Mass Spectrometry 123
Anna Drabik, Marek Noga, Marcus Macht, and Jerzy Silberring

7.6 Chemical Derivatization for Sequence Analysis 143
Adam Moszczynski, Ulf Hellman, Tomasz Dylag, Marcus Macht, and Jerzy Silberring

8 Protein Modifications 153
Simone König

9 Microfluidics 181
Agnieszka Kraj and Jerzy Silberring

10 Proteomics 187

10.1 Quantitative Proteomics 189
Tomasz Dylag, Anna Drabik, and Jerzy Silberring

10.2 Structural Proteomics 199
Malgorzata Rzychon

10.3 Functional Proteomics 217
Tomasz Dylag, Anna Drabik, and Adam Moszczynski

10.4 Clinical Proteomics 233
Anna Bodzon-Kulakowska

11 Bioinformatics 247
Pawel Ciborowski and Hesham Ali

Appendix A: Laboratory Exercises 275
Anna Drabik, Anna Bierczynska-Krzysik, Anna Bodzon-Kulakowska, and Adam Moszczynski

Appendix B: Data Tables 293

Appendix C: Internet Resources 299
Filip Sucharski, Justyna Jarzebinska, and Hana Raoof

Index 305

Über Autor(innen)

Agnieszka Kraj, PHD, is an Associate Professor of the Neurobiochemistry Department in the Faculty of Chemistry at Jagiellonian University, Poland.

Jerzy Silberring, PHD, is the Head of the Neurobiochemistry Department in the Faculty of Chemistry as well as the former deputy head of the Regional Laboratory for Physicochemical Analyses at Jagiellonian University, Poland. Dr. Silberring is coeditor of Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research and Mass Spectrometry: Instrumentation, Interpretation, and Applications (both from Wiley).