Korngreen Advanced Patch-Clamp Analysis for Neuroscientists

1. Auflage 2016
ISBN: 978-1-4939-3411-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 113, 350 Seiten, eBook

Reihe: Neuromethods

This volume presents current adaptations of the patch-clamp technique to neuroscience. Chapters focus on in-vivo recordings, voltage-gated channel recording and analysis, dendritic and axonal recordings, synaptic current recording and analysis, advanced fluorescent techniques, optogenetics and voltage-sensitive dye imaging, and finally channel and neuronal modeling. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Authoritative and practical, Advanced Patch-Clamp Analysis for Neuroscientists aimsto ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Korngreen, Alon

Inhaltsverzeichnis

1. In Vivo Whole-Cell Recordings Bojana Kokinovic, Stylianos Papaioannou, and Paolo Medini 2. Juxtasomal Loose-Patch Recordings in Awake, Head-Fixed Rats to Study the Link between Structure and Function of Individual Neurons Christiaan P.J. de Kock 3. Studying Sodium Channel Gating In Heterologous Expression Systems Jannis E. Meents and Angelika Lampert 4. Elucidating the Link between Structure and Function of Ion Channels and Transporters with Voltage-Clamp and Patch-Clamp Fluorometry Giovanni Zifarelli and Jana Kusch 5. Dendrites: Recording from Fine Neuronal Structures Using Patch-Clamp and Imaging Techniques Sonia Gasparini and Lucy M Palmer 6. Patch-clamp Recording from Myelinated Central Axons Maarten H. P. Kole and Marko Popovic 7. Analysis of Transsynaptic Attentional Neuronal Circuits with Octuple Patch-Clamp Recordings Daniel R. Wyskiel, Trevor C. Larry, Xiaolong Jiang, Guangfu Wang, and J. Julius Zhu 8. Optogenetic Dissection of the Striatal Microcircuitry Gilad Silberberg and Henrike Planert 9. Paired Recordings from Synaptically Coupled Neurones in Acute Neocortical Slices Dirk Feldmeyer and Gabriele Radnikow 10. Extracting Quantal Properties of Transmission at Central Synapses 11. Acousto-optical Scanning-Based High Speed 3D Two-Photon Imaging In Vivo Balázs Rózsa, Gergely Szalay, and Gergely Katona 12. Intracellular Voltage-Sensitive Dyes for Studying Dendritic Excitability and Synaptic Integration Corey D. Acker, Mandakini B. Singh, and Srdjan D. Antic 13. Modeling the Kinetic Mechanisms of Voltage-Gated Ion Channels Autoosa Salari, Marco A. Navarro, and Lorin S. Milescu 14. Recording and Hodgkin-Huxley Kinetic Analysis of Voltage-Gated Ion Channels in Nucleated Patches Mara Almog and Alon Korngreen 15. Creating and Constraining Compartmental Models of Neurons Using Experimental Data Stefanos S. Stefanou, George Kastellakis, and Panayiota Poirazi