Kolpashchikov / Gerasimova Nucleic Acid Detection

Methods and Protocols
Erscheinungsjahr 2013
ISBN: 978-1-62703-535-4
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 320 Seiten

Reihe: Springer Protocols

In Nucleic Acid Chemistry: Methods and Protocols , expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Kolpashchikov, Dmitry M.

Gerasimova, Yulia V.

Inhaltsverzeichnis

“Getting Blood from a Stone”: Ultra-Sensitive Forensic DNA Profiling of Microscopic Bio-particles Recovered from ‘Touch DNA’ Evidence.- Detecting MicroRNA in Human Cancer Tissues with Fluorescence in situ Hybridization.- MNAzyme qPCR: A Superior Tool for Multiplex qPCR.- A New, Multiplex, Quantitative Real-time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification.- Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with Universal Molecular Beacon Reporter.- SNP Analysis using a Molecular Beacon-based Operating Cooperatively (OC) Sensor.- Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification.- Rapid Detection of Brucella spp. using Loop-mediated Isothermal Amplification (LAMP).- Loop-mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms.- Detection of Mutation by Allele-specific Loop-mediated Isothermal Amplification (AS-LAMP).- DNA Detection by Cascade Enzymatic Signal Amplification.- Visual DNA Detection and SNP Genotyping using Asymmetric PCR and Split DNA Enzymes.- Detection of Single-stranded Nucleic Acids via Colorimetric Means, using G-quadruplex Probes.- Lateral Flow Biosensors for the Detection of Nucleic Acid.- Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a Wireless Bipolar Electrode.- High-throughput Label-free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging Sensor.- Graphene -PAMAM Dendrimer -Gold Nano Particle Composite for ElectrochemicalDNA Hybridization Detection.- Application of PNA Openers for Fluorescence-based Detection of Bacterial DNA.- DNA probes for FISH analysis of C-Negative Regions in Human Chromosomes.- Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary Electrophoresis.- High-Throughput Functional MicroRNA Profiling Using Recombinant AAV-Based MicroRNA Sensor Arrays.- The Use of Molecular Beacons to Detect and Quantify MicroRNA.- Sequence Specific Imaging of Influenza A mRNA in Living Infected Cells Using Fluorescent FIT-PNA.- Application of Caged Fluorescent Nucleotides to Live-cell RNA Imaging.