Klimanskaya / Lanza Embryonic Stem Cells

1. Auflage 2006
ISBN: 978-0-08-047112-9
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 418, 432 Seiten, Web PDF

Reihe: Methods in Enzymology

This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a 'cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using 'conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.* Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering * Presents the latest most innovative technologies * Addresses therapeutic relevance including FDA compliance and tissue engineering

Autoren/Hrsg.

Klimanskaya, Irina

Lanza, Robert

Inhaltsverzeichnis

1;Front Cover;1
2;Embryonic Stem Cells;4
3;Copyright Page;5
4;Dedication Page;6
5;Table of Contents;8
6;Contributors to Volume 418;10
7;Preface;14
8;Foreword;16
9;Volumes in Series;18
10;Section I: Derivation and Maintenance of Embryonic Stem Cells;42
10.1;Chapter 1: Murine Embryonic Stem Cells;44
10.1.1;Historical Overview;44
10.1.2;Factors Affecting the Efficiency of Mouse ES Cell Establishment;46
10.1.3;Factors Affecting the Contribution of Mouse ES Cell to Chimeric Embryos;47
10.1.4;Critical Events During Mouse ES Cell Establishment;48
10.1.5;Freezing of ES Cell Lines;53
10.1.6;Characterization;53
10.1.7;Protocols;54
10.1.8;Acknowledgments;60
10.1.9;References;60
10.2;Chapter 2: Bovine Embryonic Stem Cells;62
10.2.1;Introduction;63
10.2.2;Materials;65
10.2.3;Methods;65
10.2.4;References;77
10.3;Chapter 3: Avian Embryonic Stem Cells;79
10.3.1;Introduction;79
10.3.2;Selection of Culture Media and Routine Procedures;80
10.3.3;Derivation and Maintenance of Chicken Embryonic Stem Cell Lines;82
10.3.4;Genetic Modification of cES Cells;90
10.3.5;Production of Chimeric Chickens;92
10.3.6;Acquisition, Storage, and Quality Control of Eggs;100
10.3.7;Timeline of Chimera Production;101
10.3.8;Efficiency of Chimera Production Using Embryonic Stem Cells;102
10.3.9;Acknowledgments;103
10.3.10;References;103
10.4;Chapter 4: Zebrafish Embryonic Stem Cells;105
10.4.1;Introduction;105
10.4.2;Materials;107
10.4.3;Derivation of Zebrafish ES Cell Cultures;109
10.4.4;Homologous Recombination in ES cells and Selection of Colonies Carrying a Targeted Plasmid Insertion;114
10.4.5;Acknowledgments;116
10.4.6;References;117
10.5;Chapter 5: Human Embryonic Stem Cells;119
10.5.1;Introduction;119
10.5.2;Derivation of hES Cell Lines;120
10.5.3;Conclusion;130
10.5.4;Acknowledgments;130
10.5.5;References;130
10.6;Chapter 6: Embryonic Stem Cells from Morula;134
10.6.1;Introduction;134
10.6.2;Protocol for Isolation Human ES Cell Line from Morula;135
10.6.3;Characteristics of Morula-Derived hESC Lines;138
10.6.4;Protocol of Immunofluorescence Staining for TRA-2-39 or TRA-2-54 and Oct-4;144
10.6.5;Freezing Cell Lines;146
10.6.6;Protocol for Freezing Morula-Derived hESC Lines;146
10.6.7;Procedure for Freezing Human Embryonic Stem Cells;147
10.6.8;Thawing hESC Lines;148
10.6.9;Acknowledgments;148
10.6.10;References;148
10.7;Chapter 7: Embryonic Stem Cells from Single Blastomeres;149
10.7.1;Introduction;150
10.7.2;Derivation of ES Cells from a Single Blastomere;151
10.7.3;Analysis of Cell Lines;154
10.7.4;References;157
10.8;Chapter 8: Embryonic Stem Cells from Parthenotes;158
10.8.1;Introduction;158
10.8.2;Mouse Parthenogenetic ESCs;163
10.8.3;Nonhuman Primate Parthenogenetic ESCs;165
10.8.4;The Importance of Choosing the Right Activation Protocol;167
10.8.5;Imprinting and Parthenogenesis;169
10.8.6;Protocols for Derivation of Parthenogenetic ESCs;170
10.8.7;Summary;173
10.8.8;Acknowledgments;173
10.8.9;References;173
10.9;Chapter 9: Embryonic Stem Cells Using Nuclear Transfer;176
10.9.1;Introduction;176
10.9.2;Somatic Cell Nuclear Transfer;177
10.9.3;Media Preparation;179
10.9.4;Culture Dishes and Incubator;180
10.9.5;Protocol;180
10.9.6;Derivation of ES Cells from Cloned Blastocysts;185
10.9.7;References;187
11;Section II: Differentiation of Embryonic Stem Cells;190
11.1;Chapter 10: Neural Stem Cells, Neurons, and Glia;192
11.1.1;Introduction;192
11.1.2;Protocols;193
11.1.3;Media and Reagents;206
11.1.4;Summary;208
11.1.5;Acknowledgments;209
11.1.6;References;209
11.2;Chapter 11: Retinal Pigment Epithelium;210
11.2.1;Introduction;210
11.2.2;Differentiation of hES Cells to RPE;212
11.2.3;Materials and Equipment;214
11.2.4;Harvesting RPE Cells;220
11.2.5;Culture and Properties of hES-Derived RPE;222
11.2.6;RPE Culture Protocols;222
11.2.7;Characterization of hES-RPE Cells;223
11.2.8;Concluding Remarks;227
11.2.9;Acknowledgments;227
11.2.9.1;Appendix 1;227
11.2.9.2;Appendix 2;228
11.2.10;References;232
11.3;Chapter 12: Mesenchymal Cells;235
11.3.1;Introduction;235
11.3.2;Developmental Considerations;236
11.3.3;Adult Mesenchymal Stem Cells (MSCs);237
11.3.4;Mouse ESC-D erived Mesenchymal Tissues;238
11.3.5;Mesenchymal Cells Derived from Human ESCs;239
11.3.6;Derivation, Isolation, and Differentiation of hESC-Derived Mesenchymal Precursors;240
11.3.7;Summary and Perspectives for the Future;245
11.3.8;References;246
11.4;Chapter 13: Hematopoietic Cells;249
11.4.1;Introduction;250
11.4.2;Methods;260
11.4.3;References;277
11.5;Chapter 14: Hematopoietic Cells from Primate Embryonic Stem Cells;284
11.5.1;Introduction;284
11.5.2;Protocol for Propagation of Rhesus Monkey ES Cell Lines;286
11.5.3;Induction of Hematopoietic Differentiation of Rhesus Monkey ES Cells;288
11.5.4;Technical Notes;291
11.5.5;References;291
11.6;Chapter 15: Vascular Cells;293
11.6.1;Derivation and Characterization of Embryonic Stem (ES) Cells;293
11.6.2;Differentiation of Embryonic Stem Cells;294
11.6.3;Development of the Blood Vascular System;295
11.6.4;Vascular Developmental Potential of Embryonic Stem Cells;296
11.6.5;Vascular Differentiation of ES Cells In Vitro;297
11.6.6;References;305
11.7;Chapter 16: Cardiomyocytes;308
11.7.1;Introduction;308
11.7.2;Embryoid Body (EB) Generation;309
11.7.3;Hanging Drop Method;310
11.7.4;Human ES Cell Suspension Culture;313
11.7.5;Scalable Production of EBs in a Bioreactor;313
11.7.6;Cardiomyocyte Differentiation;319
11.7.7;Enrichment of Cardiomyocytes;320
11.7.8;Conclusion;322
11.7.9;References;323
11.8;Chapter 17: Oocytes;325
11.8.1;Introduction;325
11.8.2;General Technical Comments;329
11.8.3;Embryonic Stem Cell Culture;330
11.8.4;MEF Removal from ESC Cultures;331
11.8.5;ESC Differentiation and the Emergence of Germ Cells;331
11.8.6;Detection of Early Stage Germ Cells;332
11.8.7;Detection of Late Stage Germ Cells;333
11.8.8;Formation of Follicle-Like Structures;333
11.8.9;Oocyte Growth;336
11.8.10;Oocyte Maturation;337
11.8.11;Cleavage Stage Embryos;337
11.8.12;Reoccurring Events;338
11.8.13;Schematic Overview of the In Vitro Derivation of Oocytes from Murine ESCs;338
11.8.14;Conclusions;344
11.8.15;Acknowledgments;345
11.8.16;References;345
11.9;Chapter 18: Male Germ Cells;348
11.9.1;Introduction;348
11.9.2;Methods;349
11.9.3;Discussion;353
11.9.4;Acknowledgment;354
11.9.5;References;354
11.10;Chapter 19: Insulin-Producing Cells;356
11.10.1;Introduction;356
11.10.2;Materials and Methods;359
11.10.3;Results;370
11.10.4;Summary;371
11.10.5;Acknowledgments;371
11.10.6;References;372
11.11;Chapter 20: Pulmonary Epithelium;374
11.11.1;Introduction;374
11.11.2;Development and Organization of Pulmonary Epithelium;375
11.11.3;Lung Stem Cells;376
11.11.4;Circulating Stem Cells;377
11.11.5;In Vitro Generation of Pulmonary Epithelium from Stem Cells;378
11.11.6;References;385
12;Author Index;392
13;Subject Index;424