Kianian Plant Cytogenetics
1. Auflage 2016
ISBN: 978-1-4939-3622-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
Methods and Protocols
E-Book, Englisch, 198 Seiten
Reihe: Springer Protocols
ISBN: 978-1-4939-3622-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
This volume covers a
range of methods used in plant cytogenetics, beginning with basic analysis of
chromosomes and visualizing gene locations, to manipulating and dissecting
chromosomes, and then focusing on less understood features of chromosomes such
as recombination initiation sites and epigenomic marks. The methods described
in
Plant Cytogenetics: Methods and Protocols
build on each other and
provide, those new to the field, with a comprehensive platform to support their
research endeavours, while also introducing advanced techniques to experienced
researchers. Written in the highly successful
Methods in Molecular Biology
series
format, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting
edge and thorough,
Plant Cytogenetics: Methods and Protocols,
is a
valuable resource for anyone who is interested in the diverse and wonderfully
complex field of cytogenetics.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
C-Banding of Plant Chromosomes.- Chromosome Painting by GISH and Multi-Color FISH.- Fluorescent In Situ Hybridization on Extended Chromatin Fibers for High-Resolution Analysis of Plant Chromosomes.- Tyramide Signal Amplification: Fluorescence In Situ Hybridization for Identifying Homologous Chromosomes.- Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH.- Immunolabeling and In Situ Labelling of Isolated Plant Interphase Nuclei.- Manipulation of Homologous Chromosome Recombination in Wheat.- Dissecting Plant Chromosomes by the Use of Ionizing Radiation.- Optical Nano-Mapping and Analysis of Plant Genomes.- Flow Sorting Plant Chromosomes.- Construction of BAC Libraries from Flow-Sorted Chromosomes.- The Chromosome Microdissection and Microcloning Technique.- Immunolocalization on Whole Anther Chromosome Spreads for Male Meiosis.- Mapping Recombination Initiation Sites Using Chromatin Immunoprecipitation.- Chromatin Immunoprecipitation to Study Plant Epigenome.
