Keohavong / Grant Molecular Toxicology Protocols

2. Auflage 2014
ISBN: 978-1-62703-739-6
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1105, 646 Seiten, eBook

Reihe: Methods in Molecular Biology

Molecular Toxicology Protocols, Second Edition aims to bring together a series of articles describing validated methods to elucidate specific molecular aspects of toxicology, the emphasis being on the application of molecular methods to genetic toxicology. The volume is divided into ten parts, roughly corresponding to the spectrum of biomarkers intermediate between exposure and disease outcomes as proposed in molecular epidemiology models. Subjects of these new chapters range from preparation of fluid specimens for analysis of cellular inflammatory responses to genotoxic insults to sensitive methods for proteomic analysis and aberrant DNA methylation patterns. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Molecular Toxicology Protocols, Second Edition addresses not only the needs of molecular biologists and toxicologists, but also those of individuals interested in applying molecular methods to clinical applications, such as geneticists, pathologists, biochemists, and epidemiologists.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Keohavong, Phouthone

Grant, Stephen G.

Inhaltsverzeichnis

Array-Based Immunoassays with Rolling-Circle Amplification Detection.- Analysis of Protein Changes Using Two Dimensional Difference Gel Electrophoresis.- Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage.- Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System.- Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques.- Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR).- Strategies for Measurement of Biotransformation Enzyme Gene Expression.- Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening.- TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material.- 32P-Postlabelling Analysis of DNA Adducts.- Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot.- DNA Isolation and Sample Preparation for Quantitation of Adduct Levels by Accelerator Mass Spectrometry.- Analysis of DNA Strand Cleavage at Abasic Sites.- Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes.- Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk.- Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair.- Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice.- The Blood-Vased Glycophorin A Human In vivo Somatic Mutation Assay.- Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice.- Analysis of in Vivo Mutation at the HPRT and TK Genes of Mouse Lymphocytes.- Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems.- The Human T-CellCloning Assay: Identifying Genotypes Susceptible to Drug Toxicity and Somatic Mutation.- Molecular Analysis of HPRT Mutations in Human Lymphocytes.- Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes.- Mutation Screening of the TP53 Gene by Temporal Temperature Gel Electrophoresis.- Detection of Point Mutations of K-ras Oncogene and p53 Tumor Suppressor Gene in Sputum Samples.- ACB-PCR Quantification of Somatic Oncomutations.- Gel-Based Nonradioactive Single Strand Conformational Polymorphism and Mutation Detection.- Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesion.- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR.- Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells.- The Sister Chromatid Exchange (SCE) Assay.- The Gene Cluster Instability (GCI) Assay for Recombination.- Measuring Recombination Proficiency in Mouse Embryonic Stem Cells.- Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery.- Unscheduled DNA Synthesis: The Clinical and Functional Assay for Global Genomic Nucleotide Excision Repair.- An Immunoassay for Measuring Repair of UV Photoproducts.- Analysis of Double-Strand Break Repair by Non-Homologous DNA End Joining in Cell-Free Extracts from Mammalian Cells.- Bioenergetic Analysis of Intact Mammalian Cells Using the Seahorse XF42 Extracellular Flux Analyzer and a Luciferase ATP Assay.- Quantification of Selective Phosphatidylserine Oxidation During Apoptosis.- Quantitative Method of Measuring Phophatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron.- Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.