Keohavong / Grant | Molecular Toxicology Protocols | Buch | 978-1-58829-084-7 | sack.de

Buch, Englisch, Band 291, 490 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 1810 g

Reihe: Methods in Molecular Biology

Keohavong / Grant

Molecular Toxicology Protocols


1. Auflage 2004
ISBN: 978-1-58829-084-7
Verlag: Humana Press

Buch, Englisch, Band 291, 490 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 1810 g

Reihe: Methods in Molecular Biology

ISBN: 978-1-58829-084-7
Verlag: Humana Press


A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.

Keohavong / Grant Molecular Toxicology Protocols jetzt bestellen!

Zielgruppe


Research

Weitere Infos & Material


Part I. Analysis of DNA Adducts

32P-Postlabeling Analysis of DNA Adducts

David H. Phillips, Alan Hewer, and Volker M. Arlt

Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot

Masako Ochiai, Takashi Sugimura, and Minako Nagao

DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry

Karen H. Dingley, Esther A. Ubick, John S. Vogel, and Kurt W. Haack

Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles

Iovanna Pandelova, Stephen R. Hewitt, and John B. Hays

Analysis of DNA Strand Cleavage at Abasic Sites

Walter A. Deutsch and Vijay Hegde

Part II. Detection of Chromosomal and Genome-Wide Damage

Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes

Pataje G. S. Prasanna and William F. Blakely

Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk

Xifeng Wu, Yun-Ling Zheng, and T. C. Hsu

Flow Cytometric Analysis of Micronuclei in Erythrocytes, Jan Grawé

The Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA Damage

Günter Speit and Andreas Hartmann

Computerized Image Analysis Software for the Comet Assay

R. C. Chaubey

The Comet-FISH Technique: A Tool for Detection of Specific DNA Damage and Repair

Alexander Rapp, Michael Hausmann, and Karl Otto Greulich

DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis

Nina Joshi and Stephen G. Grant

Part III. Detection and Characterization of Surrogate Gene Mutation

Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes

Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich

Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems

Roy R. Swiger

Methods for Detecting Somatic Mutations In Vitro: The Human T-Cell Cloning Assay Selecting for HPRT Mutants

Sai-Mei Hou

Molecular Analysis of Mutations in the Human HPRT Gene

Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant

Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes

James C. Fuscoe

The GPA In Vivo Somatic Mutation Assay

Stephen G. Grant

Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR: Detecting Somatic Mutations in Humans and Mice

Seishi Kyoizumi, Yoichiro Kusunoki, and Tomonori Hayashi

Part IV. Detection and Characterization of Cancer Gene Mutation

Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis

Therese Sørlie, Hilde Johnsen, Phuong Vu, Guro Elisabeth Lind, Ragnhild Lothe, and Anne-Lise Børresen-Dale

Analysis of K-RAS and P53 Mutations in Sputum Samples

Weimin Gao and Phouthone Keohavong

Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution

Barbara L. Parsons, Page B. McKinzie, and Robert H. Heflich

Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection: Limitations and Solutions

Vibhuti Gupta, Reetakshi Arora, Anand Ranjan, Narendra K. Bairwa, Dheeraj K. Malhotra, P. T. Udhayasuriyan, Anjana Saha, and Ramesh Bamezai

Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions

Toshinari Minamoto

Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR

Michael J. Villalobos, Christopher J. Betti, and Andrew T. M. Vaughan

Part V. Analysis of DNA Repair Mechanisms

Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery

Anjana Saha, Narendra K. Bairwa, and Ramesh Bamezai

Unscheduled DNA Synthesis: A Functional Assay for Global Genomic Nucleotide Excision Repair

Crystal M. Kelly and Jean J. Latimer



Ihre Fragen, Wünsche oder Anmerkungen
Vorname*
Nachname*
Ihre E-Mail-Adresse*
Kundennr.
Ihre Nachricht*
Lediglich mit * gekennzeichnete Felder sind Pflichtfelder.
Wenn Sie die im Kontaktformular eingegebenen Daten durch Klick auf den nachfolgenden Button übersenden, erklären Sie sich damit einverstanden, dass wir Ihr Angaben für die Beantwortung Ihrer Anfrage verwenden. Selbstverständlich werden Ihre Daten vertraulich behandelt und nicht an Dritte weitergegeben. Sie können der Verwendung Ihrer Daten jederzeit widersprechen. Das Datenhandling bei Sack Fachmedien erklären wir Ihnen in unserer Datenschutzerklärung.