Practical Protein Chromatography

1992. Auflage 1992
ISBN: 978-0-89603-213-2
Verlag: Humana Press

Buch, Englisch, Band 11, 327 Seiten, Format (B × H): 157 mm x 234 mm, Gewicht: 748 g

Reihe: Methods in Molecular Biology

Kenney and Fowell's exceptionally useful new book, Practical Protein Chromatography, brings all the forefront methods of protein separation and purification directly to your laboratory benchtop. The book treats not only powerful analytical separations, but also the small- and large-scale preparative techniques that are currently applicable throughout biological and biomedical research.

Chapters, written by experts in each of the techniques covered, treat a variety of methods usable in virtually every laboratory with a chromatographic system. Topics include: immunoaffinity chromatography and selection of antibodies • lectin affinity and dye-ligand chromatography • exploiting weak affinities • alternative coupling chemistries • biospecific affinity elution • size-exclusion HPLC of proteins • chromatofocusing • determination of purity and yield • specialized chromatographic techniques.

The established tradition of Methods in Molecular Biology is to present techniques that guarantee optimum results. Everyone doing protein chromatography today-at any level of proficiency-will find Practical Protein Chromatography an indispensable, hands-on guide to the effective use of these methodologies.

Zielgruppe

Research

Autoren/Hrsg.

Kenney, Andrew

Fowell, Susan

Fachgebiete

• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Naturwissenschaften Biowissenschaften Proteinforschung

Inhaltsverzeichnis

Thiophilic Adsorption Chromatography.- Immobilized Metal Ion Affinity Chromatography.- Histidine Ligand Affinity Chromatography.- Boronic Acid Matrices for the Affinity Purification of Glycoproteins and Enzymes.- Calcium-Dependent Hydrophobic Interaction Chromatography.- Lectin Affinity Chromatography.- Dye-Ligand Affinity Chromatography.- High-Performance Affinity Chromatography for Protein Separation and Purification.- Immunoafflnity Chromatography.- Selection of Antibodies for Immunoaffinity Chromatography.- Some Alternative Coupling Chemistries for Affinity Chromatography.- Exploiting Weak Affinities.- Biospecific Affinity Elution.- Size-Exclusion High-Performance Liquid Chromatography of Proteins.- Chromatofocusing.- Ion-Exchange Chromatography of Proteins.- Displacement Chromatography of Proteins.- Purification of DNA Binding Proteins by Affinity and Ion Exchange Chromatography.- Making Bacterial Extracts Suitable for Chromatography.- Determination of Purity and Yield.