RNA Abundance Analysis

Methods and Protocols
2. Auflage 2021
ISBN: 978-1-0716-0742-8
Verlag: Springer US

Buch, Englisch, Band 2170, 233 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 671 g

Reihe: Methods in Molecular Biology

This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Kaloshian, Isgouhi

Jin, Hailing

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana.- Strand-Specific RNA-Seq Applied to Malaria Samples.- Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning.- Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting.- Quantitative Analysis of Plant miRNA Primary Transcripts.- A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq.- Analysis of RBP Regulation and Co-Regulation of mRNA 3’ UTR Regions in a Luciferase Reporter System.- Extraction of Small RNAs by Titanium Dioxide Nanofibers.- Identification of MicroRNAs and Natural Antisense Transcript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data.- Purification and Analysis of Chloroplast RNAs in Arabidopsis.- In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes.- Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs.- Isolation of Insect Bacteriocytes as a Platform for Transcriptomic Analyses.- Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue.- Studying RNA-Protein Interaction Using Riboproteomics.- Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequential Protoplastation.