Proteomics in Functional Genomics

Protein Structure Analysis
Erscheinungsjahr 2012
ISBN: 978-3-0348-9576-7
Verlag: Birkhäuser

Buch, Englisch, 236 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 394 g

Reihe: Experientia Supplementum

A wealth of information has accumulated over the last few years on the

human genome. The new insights have completely changed the focus of

protein analysis. It is no longer time-consuming analysis of unknown

products, but rather selective identifications of individual forms,

modifications and processings, and overall analysis of global protein

outputs from cells and tissues in health and disease.

This book gears to the rising need of sensitive, accurate, and fast

separation and identification techniques in proteomics. It discusses

current methodologies of modern protein analysis, from isolation and

sample preparation, over analysis and identification, to final

characterization. Several evaluations concentrate on the now productive

approaches of two-dimensional gel electrophoresis and mass spectrometry,

but alternative methods and further perspectives are also outlined. The

book includes an overlook over current databases to connect protein

analysis data with all available information,.

Zielgruppe

Research

Autoren/Hrsg.

Jolles, P.

Jörnvall, Hans

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien

Inhaltsverzeichnis

Proteome mapping by two-dimensional polyacrylamide gel electrophoresis in combination with mass spectrometric protein sequence analysis.- Techniques for sample preparation including methods for concentrating peptide samples.- Sample preparation by SDS/PAGE and in-gel digestion.- High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection.- Nanospray mass spectrometry in protein and peptide chemistry.- MALDI-TOF mass spectrometry in protein chemistry.- The chemistry of protein sequence analysis.- The alkylated thiohydantoin method for C-terminal sequence analysis.- Ladder Sequencing.- New approaches for innovations in sensitive Edman sequence analysis by design of a wafer-based chip sequencer.- Analysis of glycoprotein-derived glycopeptides.- Lipopeptide preparation and analysis.- Phosphopeptide analysis.- Bioinformatics in protein analysis.