Gap Junction Protocols

Softcover Nachdruck of the original 1. Auflage 2016
ISBN: 978-1-4939-8110-6
Verlag: Springer

Buch, Englisch, Band 1437, 235 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 4801 g

Reihe: Methods in Molecular Biology

Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Johnstone, Scott R.

Vinken, Mathieu

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Analysis of Liver Connexin Expression
Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction.- DNA Methylation Analysis of Human
Tissue-Specific Connexin Genes.- Detection of Connexins in Liver Cells Using
Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis.- Immunohisto- and
Cytochemistry Analysis of Connexins.- Small Interfering RNA-Mediated
Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells.- Generation and Use of Trophoblast
Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy
and Labor.- Identification of Connexin43
Phosphorylation and S-Nitrosylation
in Cultured Primary Vascular Cells.- Preparation of Gap Junctions in
Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome
Analysis.- Scrape Loading/Dye Transfer Assay.- Microinjection Technique for Assessment of Gap
Junction Function.- Electroporation Loading and Dye
Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling.- Using Fluorescence Recovery After Photobleaching to Study Gap Junctional
Communication In Vitro.- Tracking Dynamic Gap Junctional Coupling in Live Cells by Local
Photo-Activation and Fluorescence Imaging.- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of
Second Messengers in Cultured Bone Cells.- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method
for Studying Gap Junctions and Hemichannels.- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for
the Measurement of Gap Junction Conductance.