Johnson / Waterman Cytochrome P450, Part C

1. Auflage 2002
ISBN: 978-0-08-049702-0
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 421 Seiten, Web PDF

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.
Key Features
* Human Genomics and Genetics
* Structure and Mechanism
* Regulation of Expression
* Metabolism
* Invertibrate P450s

Autoren/Hrsg.

Johnson, Eric F.

Waterman, Michael R.

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 357;10
4;Preface;16
5;Volume in Series;18
6;Section I: Human Genomics and Genetics;39
6.1;Chapter 1. Mining Databases for Cytochrome P450 Genes;41
6.2;Chapter 2. Sequence Alignments, Variabilities, and Vagaries;53
6.3;Chapter 3. Human CYP Allele Database: Submission Criteria Procedures and Objectives;66
6.4;Chapter 4. Fine-Scale Mapping of CYP Gene Clusters: An Example from Human CYP4 Family;74
6.5;Chapter 5. Detection of Single Nucleotide Polymorphisms in CYP2B6 Gene;83
6.6;Chapter 6. Genotyping Human Cytochrome: P450 1B1 Variants;91
6.7;Chapter 7. Genotyping Human CYP2A6 Variants;97
7;Section II: Structure and Mechanism;109
7.1;Chapter 8. Purification and Crystallization of N-Terminally Truncated Forms of Microsomal Cytochrome P450 2C5;111
7.2;Chapter 9. Molecular Replacement in P450 Crystal Structure Determinations;117
7.3;Chapter 10. Optical Biosensor and Scanning Probe Microscopy Studies of Cytochrome P450 Interactions with Redox Partners and Phospholipid Layers;132
7.4;Chapter 11. Cryoradiolysis for the Study of P450 Reaction Intermediates;141
7.5;Chapter 12. Analyzing Binding of N-Terminal Truncated, Microsomal Cytochrome P450s to Membranes;154
7.6;Chapter 13. Sensitizer-Linked Substrates and Ligands: Ruthenium Probes of Cytochrome P450 Structure and Mechanism;158
7.7;Chapter 14. Combining Pharmacophore and Protein Modeling to Predict CYP450 Inhibitors and Substrates;171
7.8;Chapter 15. High Pressure: A New Tool to Study P450 Structure and Function;183
8;Section III: Regulation of Expression;197
8.1;Chapter 16. Use of in Vitro Pregnane X Receptor Assays to Assess CYP3A4 Induction Potential of Drug Candidates;199
8.2;Chapter 17. Analysis of CYP mRNA Expression by Branched DNA Technology;208
8.3;Chapter 18. Ligand-Induced Coactivator Recruitment to Peroxisome Proliferator-Activated Receptora Characterized by Fluorescence Resonance Energy Transfer;218
8.4;Chapter 19. Fluorescence-Based Ligand-Binding Assays for Peroxisome Proliferator-Activated Receptors;226
8.5;Chapter 20. Developing Toxicologically Predictive Gene Sets Using cDNA Microarrays and Bayesian Classification;236
8.6;Chapter 21. Direct Expression of Fluorescent Protein-Tagged Nuclear Receptor CAR in Mouse Liver;243
8.7;Chapter 22. Application of Fluorescent Differential Display and Peroxisome Proliferator-Activated Receptor (PPAR)a-Null Mice to Analyze PPAR Target Genes;252
8.8;Chapter 23. Histological and Metabolism Analysis of P450 Expression in the Brain;279
8.9;Chapter 24. Proteomic Analysis of Rodent Hepatic Responses to Peroxisome Proliferators;287
9;Section IV: Metabolism;297
9.1;Chapter 25. Kinetic Analysis for Multiple Substrate Interaction at the Active Site of Cytochrome P450;299
9.2;Chapter 26. Design and Application of Fluorometric Assays for Human Cytochrome P450 Inhibition;314
9.3;Chapter 27. Automated Quantitative and Qualitative Analysis of Metabolic Stability:AProcess for Compound Selection during Drug Discovery;323
9.4;Chapter 28. Characterization of Covalent Adducts to Intact Cytochrome P450s by Mass Spectrometry;334
9.5;Chapter 29. Mutagenesis Testing Based on Bacterial Expression of Human P450s;339
9.6;Chapter 30. Use of Long-Term Cultures of Human Hepatocytes to Study Cytochrome P450 Gene Expression;349
9.7;Chapter 31. Polarized Cell Cultures for Integrated Studies of Drug Metabolism and Transport;359
10;Section V: Invertebrate P450s;369
10.1;Chapter 32. Use of Methylotropic Yeast Pichia pastoris for Expression of Cytochromes P450;371
10.2;Chapter 33. Partial Recoding of P450 and P450 Reductase cDNAs for Improved Expression in Yeast and Plants;381
10.3;Chapter 34. Selective Covalent Labeling with Radiolabeled Suicide Substrates for Isolating P450s;390
10.4;Chapter 35. Cloning of cDNAs Encoding P450s in Flavonoid/ Isoflavonoid Pathway from Elicited Leguminous Cell Cultures;398
10.5;Chapter 36. Selected Cell Cultures and Induction Methods for Cloning and Assaying Cytochromes P450 in Alkaloid Pathways;408
10.6;Chapter 37. Isolation and Functional Characterization of Cytochrome P450s in Gibberellin Biosynthesis Pathway;419
11;Author Index;427
12;Subject Index;449