Jain / Saxena Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

2009
ISBN: 978-1-60327-287-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 547, 411 Seiten, eBook

Reihe: Methods in Molecular Biology

Given the vital and far-reaching applications of medicinal plant metabolites worldwide, the quality and consistency of the products as well as the very survival of various species are of the utmost importance. In , expert researchers provide detailed, step-by-step protocols for the establishment of cultures of key medicinal plants, their mass multiplication in a controlled environment, and step-wise secondary metabolite analysis, genetic transformation, large-scale metabolite production in a bioreactor, and molecular markers. In addition, many of these protocols will provide a basis for much needed efforts of germplasm conservation or cryopreservation of medicinal plant species at the brink of extinction as well as efforts to protect them from the adverse impact of rapid climatic changes. As a volume in the ™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, is an ideal resource for scientists endeavoring to continue the research on this exciting natural branch of medicine.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Jain, Shri Mohan

Saxena, Praveen K.

Inhaltsverzeichnis

Section A: Micropropagation and Chemical Analysis

1. Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives

Kee Yoeup Paek, Hosakatte Niranjana Murthy, and Eun Joo Hahn

2. In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium, Assessment of Genetic Fidelity of Micropropagated Plants, and Simultaneous Quantization of Reserpine, Ajmaline and Ajmalicine

M. K. Goel, S. Mehrotra, A. K. Kukreja, K. Shanker, and S.P.S. Khanuja

3. Medicinal Properties, in vitro Protocols and Secondary Metabolite Analyses of Scots Pine
Hely Häggman, Anna Maria Pirttilä, Karoliina Niemi, Tytti Sarjala, and Riitta Julkunen-Tiitto

4. Saussurea medusa Cell Suspension Cultures for Flavonoid Production
Chun-Zhao Liu and Praveen K. Saxena

5. Micropropagation of Crataeva nurvala

Shashi B. Babbar, Neetika Walia, and Amandeep Kaur

6. Bilberry In vitro Protocols and Analyses of Phenolic Compounds

Laura Jaakola, Kaisu Riihinen, Hely Häggman, and Anja Hohtola

7. In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana
S. Pillai Geetha, AV. Raghu, Gerald Martin, Satheesh George, and Indira Balachandran

8. In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R.Br. Ex Roemer & Schultes through Callus Culture Under Abiotic Stress Conditions

Abdul Bakrudeen Ali Ahmed, Adhikarla Suryanarayana Rao, and Mandali Venkateswara Rao

9. Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis

Suk Weon Kim and Myung Jin Oh

10. In vitro Culture and Secondary Metabolite Isolation in Bryophytes
Aneta Sabovljevic, Marko Sabovljevic, and NebojsaJockovic

11. Micropropagation and In vitro Conservation of Vanilla (Vanilla planifolia Andrews.)

Minoo Divakaran and K. Nirmal Babu

12. Protocol for In vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham

Sushma Kaul, K.A. Suri, P. Dutt, M. Sambyal, A. Ahuja, and M.K. Kaul

13. Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria

Ian B. Cole, Faisal T. Farooq, and Susan J. Murch

14. Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae)

Elizabete Catapan, Fábio Netto Moreno, Márcio Luís Busi da Silva, Michel Fleith Otuki, Rivaldo Niero, Valdir Cechinel Filho, Rosendo Augusto Yunes, and Ana Maria Viana

15. In vitro Clonal Propagation of Asparagus racemosus, a High Value Medicinal Plant

Sanjay Saxena and Nishritha Bopana

16. Micropropagation of Penthorum chinense through Axillary Bud

Jun Yang and Zheng-song Peng

Section B: Transgenic Approaches

17. Spontaneous Plant Regeneration and Production of Secondary Metabolites from Hairy Root Cultures of Centaurium erythraea Rafn

Angelina Subotic, Sladana Jevremovic, Dragoljub Grubišic, Teodora Jankovic

18. Transgenic Hypericum perforatum

G. Franklin, Margarida M. Oliveira, and Alberto C.P. Dias

19. Agrobacterium-Mediated Transformation of Ruta graveolens L.

Karine Lièvre, Thi Lê Minh Tran, Sébastien Doerper, Alain Hehn, Paul Lacoste, Brigitte Thomasset, Frédéric Bourgaud, and Eric Gontier

20. Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures

Katarína Brunáková and Ján Košuth

21. Catapol Production in Chinese Foxglove (Rehmannia glutinosa Libos.) Hairy Roots Transformed with