Iyengar G Protein Pathways, Part C: Effector Mechanisms

1. Auflage 2001
ISBN: 978-0-08-049693-1
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: 6 - ePub Watermark

E-Book, Englisch, Band Volume 345, 653 Seiten

Reihe: Methods in Enzymology

This third volume in the trio covering G proteins, features integrated approaches to studying G proteins. Methods pertaining to signaling mechanisms are presented, including theoretical and modeling approaches, biochemistry and molecular biology, and cell biology and physiology. The techniques for studying the structure and function of G proteins are important not only to those with specific research interests in them, but also endocrinologists and pharmacologists conducting research on signaling mechanisms that are increasingly understood to interact with G proteins.

Autoren/Hrsg.

Iyengar, Ravi

Inhaltsverzeichnis

1;Front Cover;1
2;G Protein Pathways;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors to Volume 345;12
6;Preface;18
7;Volume in Series;22
8;Section I: Modeling Intracellular Signaling Pathways;42
8.1;Chapter 1. Use of Kinetikit and GENESIS for Modeling Signaling Pathways;44
9;Section II: Phosphodiesterases;66
9.1;Chapter 2. Assays of G Protein/cGMP–Phosphodiesterase Interactions;68
9.2;Chapter 3. Assaying G Protein–Phosphodiesterase Interactions in Sensory Systems;78
10;Section III: Calcium and Potassium Channels;90
10.1;Chapter 4. Studies of Endogenous G-Protein-Mediated Pathways in Neurons by Whole-Cell Electrophysiology;92
10.2;Chapter 5. Biochemical Approaches to Study Interaction of Calcium Channels with RGS12 in Primary Neuronal Cultures;101
10.3;Chapter 6. Assaying Phosphatidylinositol Bisphosphate Regulation of Potassium Channels;112
11;Section IV: Adenylyl Cyclases;134
11.1;Chapter 7. Purification of Soluble Adenylyl Cyclase;136
11.2;Chapter 8. Calcium-Sensitive Adenylyl Cyclase/Aequorin Chimeras as Sensitive Probes for Discrete Modes of Elevation of Cytosolic Calcium;146
11.3;Chapter 9. Kinetic Analysis of the Action of P-Site Analogs;153
11.4;Chapter 10. Expression, Purification, and Assay of Cytosolic (Catalytic) Domains of Membrane-Bound Mammalian Adenylyl Cyclases;168
11.5;Chapter 11. Identification of Putative Direct Effectors for G?o, Using Yeast Two-Hybrid Method;181
11.6;Chapter 12. Identification of Transmembrane Adenylyl Cyclase Isoforms;191
11.7;Chapter 13. Functional Analyses of Type V Adenylyl Cyclase;201
11.8;Chapter 14. Photoaffinity Labeling of Adenylyl Cyclase;229
11.9;Chapter 15. Crystallization of Complex between Soluble Domains of Adenylyl Cyclase and Activated Gs?;239
11.10;Chapter 16. Generation of Adenylyl Cyclase Knockout Mice;247
11.11;Chapter 17. Construction of Soluble Adenylyl Cyclase from Human Membrane-Bound Type 7 Adenylyl Cyclase;272
11.12;Chapter 18. Genetic Selection of Regulatory Mutants of Mammalian Adenylyl Cyclases;282
12;Section V: Phospholipases and Lipid-Derived Products;294
12.1;Chapter 19. Expression and Characterization of Rat Brain Phospholipase D;296
12.2;Chapter 20. G-Protein-Coupled Receptor Regulation of Phospholipase D;306
12.3;Chapter 21. Analysis and Quantitation of Ceramide;316
12.4;Chapter 22. Assays for Phospholipase D Reaction Products;335
12.5;Chapter 23. Determination of Strength and Specificity of Membrane-Bound G Protein–Phospholipase C Association Using Fluorescence Spectroscopy;347
12.6;Chapter 24. Assays and Characterization of Mammalian Phosphatidylinositol 4,5-Bisphosphate-Sensitive Phospholipase D;369
12.7;Chapter 25. Characterization and Purification of Phosphatidylinositol Trisphosphate 5-Phosphatase from Rat Brain Tissues;376
13;Section VI: Small GTP-Binding Proteins;388
13.1;Chapter 26. Assay of Cdc42, Rac, and Rho GTPase Activation by Affinity Methods;390
13.2;Chapter 27. Assays of ADP-Ribosylation Factor Function;400
13.3;Chapter 28. Functional Characterization of p115 RhoGEF;412
13.4;Chapter 29. Nonisotopic Methods for Detecting Activation of Small G Proteins;424
13.5;Chapter 30. BIG1 and BIG2: Brefeldin A-Inhibited Guanine Nucleotide-Exchange Proteins for ADP Ribosylation Factors;438
13.6;Chapter 31. Functional Interaction of G?13 with p115RhoGEF Determined with Transcriptional Reporter System;445
14;Section VII: Protein Kinases and Phosphatases;452
14.1;Chapter 32. Analysis of c-Jun N-Terminal Kinase Regulation and Function;454
14.2;Chapter 33. Double-Label Confocal Microscopy of Phosphorylated Protein Kinases Involved in Long-Term Potentiation;467
14.3;Chapter 34. Regulation of Mitogen-Activated Protein Kinases by G-Protein-Coupled Receptors;478
14.4;Chapter 35. Analysis of Protein Kinase B/Akt;489
14.5;Chapter 36. Direct Stimulation of Bruton’s Tyrosine Kinase by G Protein ? Subunits;505
14.6;Chapter 37. Isozyme-Specific Inhibitors and Activators of Protein Kinase C;511
14.7;Chapter 38. Assay of Raf-1 Activity;531
14.8;Chapter 39. Analyzing Protein Kinase C Activation;540
14.9;Chapter 40. Assays for Protein-Tyrosine Phosphatases;548
14.10;Chapter 41. Differential Display of mRNAs Regulated by G-Protein Signaling;562
14.11;Chapter 42. Gene Profiling of Transgenic Mice with Targeted Expression of Activated Heterotrimeric G Protein ? Subunits Using DNA Microarray;570
14.12;Chapter 43. Retroviral Vectors Applied to Gene Regulation Studies;580
14.13;Chapter 44. Overexpression of Tightly Regulated Proteins: Protein Phosphatase 2A Overexpression in NIH 3T3 Cells;592
14.14;Chapter 45. Monitoring G-Protein-Coupled Receptor Signaling with DNA Microarrays and Real-Time Polymerase Chain Reaction;597
14.15;Chapter 46. cAMP Response Element-Mediated Gene Expression in Transgenic Reporter Gene Mouse Strain;611
14.16;Chapter 47. Functional Genomic Search of G-Protein-Coupled Receptors Using Microarrays with Normalized cDNA Library;626
15;Author Index;642
16;Subject Index;676