Immuno-oncology and Immunotherapy Part G

Erscheinungsjahr 2026
ISBN: 978-0-443-29570-6
Verlag: Elsevier Science & Technology

Buch, Englisch, Format (B × H): 152 mm x 229 mm, Gewicht: 450 g

Immuno-oncology and Immunotherapy, Part G, Volume 202 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including In situ imaging of pulmonary invariant Natural Killer T cells in precision cut lung slices, Immunofluorescent microscopy to quantify and characterize chromosomal instability, Spatial techniques for understanding the tumor microenvironment, Very high-flex immunophenotyping using co-linear cytofluorometric evaluation, Large-scale CRISPR-Cas9 screens to define regulators of immune checkpoint biology, Assessment of cytoplasmic dsDNA for cellular immunogenicty, and much more.

Additional sections cover Harmonization of a multiparametric flow cytometry panel for immunological monitoring on PBMC and whole blood samples: a real-life example, Quantitative assessment of arterial remodeling from confocal microscopy image analysis of the mid-gestation mouse decidua, Phenotypic characterization and function of tumor infiltrating and peripheral B cells in cancer patients, Methodological Advances to analyze Cancer-Associated Fibroblast-derived ECM effects on Macrophage Polarization, and many other topics that will be of interest to readers.

Autoren/Hrsg.

Weitere Mitwirkende

Charpentier, Maud

Bravo-San Pedro, Jose Manuel

Fachgebiete

• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

1. In situ imaging of pulmonary invariant Natural Killer T cells in precision cut lung slices
Claudia Burrello, Karin E. de Visser and Tom Langelaar
2. Immunofluorescent microscopy to quantify and characterize chromosomal instability
Pippa F. Cosper, Kathryn Jones, Yeseo Choi, Radchanon Leelasukseree, Nikki Messick, Ava Bryan and Sophie Bice
3. Spatial techniques for understanding the tumour microenvironment
Florent Ginhoux, Margaux GARDET and Kevin Mulder
4. Very high-flex immunophenotyping using co-linear cytofluorometric evaluation
Benjamin Izar
5. Large-scale CRISPR-Cas9 screens to define regulators of immune checkpoint biology
Benjamin Izar
6. Assessment of cytoplasmic dsDNA for cellular immunogenicty
Rongze Olivia Lu, Raymond Sun, Oishika Das and Isha Mondol
7. Harmonization of a multiparametric flow cytometry panel for immunological monitoring on PBMC and whole blood samples: a real-life example
Iole Macchia Sr., Francesca Urbani and Valentina La Sorsa
8. Quantitative assessment of arterial remodeling from confocal microscopy image analysis of the mid-gestation mouse decidua
Alexander J. Muller and Dema Alghabban
9. Phenotypic characterization and function of tumor infiltrating and peripheral B cells in cancer patients
Paola Nistico, Maria Rosado, Giuseppe Frisullo, Giulia Campo and Miriam Petrella
10. Methodological Advances to analyze Cancer-Associated Fibroblast-derived ECM effects on Macrophage Polarization
Paola Nistico, Annalisa Tocci, Nicla Porciello, Belinda Palermo, Lorenzo Valenti, Anna Di Carlo and Francesca Di Modugno
11. Lymphocyte migration/invasion assays
Louis M. Weiner, Rachael Maynard and Alex Lekan
12. Flow-cytometry-based assay to study CD8+ T cell cytotoxicity against tumor cells.
Roberta Zappasodi, Alexey V Sarapulov and Marc-Anthony Rodriguez
13. High dimensional flow cytometry for conventional and unconventional T cell populations
Maria Paula Roberti, Andrea Leufgen, Conrad Rauber, Annika Steitz, Paula Daza and Maria Paz Gavilan
14. Optimized Phospho-Flow Cytometry for Quantitative Analysis of Early TCR-Proximal Signaling: Implications for T Cell Function in Onco-Immunology
Paola Nistico, Nicla Porciello, Annalisa Tocci, Giulia Campo and Belinda Palermo

Über Autor(innen)

Bravo-San Pedro, Jose Manuel
Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).

Charpentier, Maud
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models.