Peptide Conjugation

Methods and Protocols
1. Auflage 2021
ISBN: 978-1-0716-1619-2
Verlag: Springer US

Buch, Englisch, Band 2355, 324 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 634 g

Reihe: Methods in Molecular Biology

This volume explores diverse protocols for peptide conjugation, and provides thoroughly tested and scientifically valid techniques that allow researchers and scientists to prepare, purify, characterize, and use peptide conjugation methods for chemical, biochemical, and biological studies. Some of the topics discussed in this book are gold nanoparticles, proteins, pegylated lipids, and vitamins. Chapters also cover enzymatic ligation using sortase A, construction of a phage-displayed cyclic-peptide library, quantum dot-peptide conjugates, and preparation of lipopeptides by CLipPA chemistry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Cutting-edge and comprehensive, Peptide Conjugation: Methodsand Protocols is a valuable resource for experienced researches and undergraduate students alike who are interested in learning more about this exciting and developing field. 

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Hussein, Waleed M.

Toth, Istvan

Stephenson, Rachel J.

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)

Inhaltsverzeichnis

Preface…
Table of Contents…
Contributing Authors…

1. Peptide-Polymer Conjugation via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition
Waleed M. Hussein, Istvan Toth, and Mariusz Skwarczynski

2. Conjugation of Peptides to Gold Nanoparticles
Pornsuda Maraming and James Chen Yong Kah

3. Peptide-Protein Conjugation and Characterization to Develop Vaccines for Group A Streptococcus
Sharareh Eskandari, Michael F. Good, and Manisha Pandey

4. Oxime/Hydrazone Conjugation at Histidine: Late-Stage Functionalization Approach of Unprotected Peptides
Anaïs F. M. Noisier and Ranganath Gopalakrishnan

5. Modification of Nanoparticles with Transferrin for Targeting Brain Tissues
Sara Aly Attia, Xiang Li, Nina Filipczak, Daniel F. Costa, and Vladimir P. Torchilin

6. Peptide-Pegylated Lipid Conjugation Via Copper-Catalyzed Alkyne-Azide 1,3-Dipolar Cycloaddition
Waleed M. Hussein and Istvan Toth

7. Vitamin B12 - Peptide Nucleic Acid Conjugates
Aleksandra J. Wierzba, Monika Wojciechowska, Joanna Trylska, and Dorota Gryko

8. Enzymatic Ligation of Disulfide-Rich Animal Venom Peptides: Using Sortase A to Form Double-Knotted Peptides
Poanna Tran and Christina I. Schroeder

9. Solid-State, Thermal Synthesis of Peptide/Protein–Boron Cluster Conjugates
Krzysztof Fink and Tomasz M. Goszczynski

10. Bioconjugation of Peptides to Hybrid Gold Nanoparticles
Rosalba Moretta, Monica Terracciano, Ilaria Rea, and Luca De Stefano

11. Design and Synthesis of a Peptide-Based Glioma-Targeted Drug Delivery Vector gHope2
Kaido Kurrikoff, Nikita Oskolkov, Elo Eriste, and Ülo Langel

12. Electrochemically-Enabled C-Terminal Peptide Modifications
Yutong Lin and Lara R. Malins

13. Double Conjugation Using Mercapto-Acryloyl and Alkyne-Azide Reactions for the Synthesis of Branched Multiantigenic Vaccine Candidates
Waleed M. Hussein, Mariusz Skwarczynski, and Istvan Toth

14. Chemical Protein Synthesis by Chemoselective a-Ketoacid–Hydroxylamine (KAHA) Ligations with 5-Oxaproline
Jako