Buch, Englisch, Band 1742, 302 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1096 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 1742, 302 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1096 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-7664-5
Verlag: Springer
This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. Chapters guide the reader through the application of hypoxic conditions, to the techniques of molecular biology, biochemical, cell biology, genomic,bioinformatic, metabolic, and animal studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Hypoxia: Methods and Protocols aims to provide a valuable set of tools that can be used to study hypoxia and beyond.
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Weitere Infos & Material
1. Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma
Kazimierz O. Wrzeszczynski, Vanessa Felice, Minita Shah, Sadia Rahman, Anne-Katrin Emde, Vaidehi Jobanputra, Mayu Frank, and Robert B. Darnell
2. Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina BeadArrays
Jonathan Serrano and Matija Snuderl
3. Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens
Amit D. Gujar, Diane D. Mao, John B. Finlay, and Albert H. Kim
4. Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative SpecimensJoshua Frenster and Dimitris Placantonakis
5. Isolation of GBM Stem Cells with Flow Cytometry
Julio Inocencio, Joshua Frenster, and Dimitris Placantonakis
6. Lentiviral Transduction of Primary Human Glioblastoma Cultures
Joshua Frenster, Julio Inocencio, and Dimitris Placantonakis
7. Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors
N. Sumru Bayinand Dimitris G. Placantonakis
8. Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF
Nataliya Galifianakis, Dimitris G. Placantonakis, and Mitchell Chesler
9. Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro
Jean-Pierre Gagner, Mirna Lechpammer, and David Zagzag
10. Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry
Jingjing Deng, Guoan Zhang, and Thomas A. Neubert
11. Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS)
Hediye Erdjument-Bromage, Fang-Ke Huang, and Thomas A. Neubert
12. Single-Cell RNA Sequencing of Glioblastoma Cells
Rajeev Sen, Igor Dolgalev, N. Sumru Bayin, Adriana Heguy, Aris Tsirigos, and Dimitris G. Placantonakis
13. Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures
N. Sumru Bayin, Lin Ma, Dimitris Placantonakis, and Mary Helen Barcellos-Hoff
14. Orthotopic Patient-derived Glioblastoma Xenografts in Mice
Zhongye Xu, Michael Kader, Rajeev Sen, and Dimitris Placantonakis
15. Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice
Joshua Frenster and Dimitris Placantonakis
16. Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy
N. Sumru Bayinand Dimitris G. Placantonakis
17. Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection
Jean-Pierre Gagnerand David Zagzag
18. Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma
Karolina Woroniecka, Pakawat Chongsathidkiet, Aladine Elsamadicy, Harrison Farber, Xiuyu Cui, and Peter E. Fecci
19. Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages
Aram S. Modrek, Jod Prado, Devin Bready, Joravar Dhaliwal, Danielle Golub, and Dimitris G. Placantonakis
