Buch, Englisch, 262 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 404 g
Reihe: Methods in Molecular Biology
Buch, Englisch, 262 Seiten, Previously published in hardcover, Format (B × H): 152 mm x 229 mm, Gewicht: 404 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61737-490-6
Verlag: Humana Press
Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.
Zielgruppe
Research
Autoren/Hrsg.
Weitere Infos & Material
Common Strategies.- Fundamentals of Modern Peptide Synthesis.- Chimerism.- Synthetic Methodologies and Applications.- Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars.- Synthesis of Linear, Branched, and Cyclic Peptide Chimera.- Synthesis of Cell-Penetrating Peptides for Cargo Delivery.- Incorporation of Phosphotyrosyl Mimetic 4- (Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides.- Expressed Protein Ligation for Protein Semisynthesis and Engineering.- Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides.- Quenched Fluorescent Substrate- Based Peptidase Assays.- A Convenient Method for Synthesis of Cyclic Peptide Libraries.- High-Throughput Peptide Synthesis.- Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C - Terminal Modified Peptides.- 13 Synthesis of Peptide Bioconjugates.- Practical Guides.- Protein Identification by Mass Spectrometric Analyses of Peptides.- Manual Solid Phase Synthesis of Glutathione Analogs.
