Hofman / Thonart / Gembloux Engineering and Manufacturing for Biotechnology

1. Auflage 2006
ISBN: 978-0-306-46889-6
Verlag: Springer Netherlands
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 4, 496 Seiten

Reihe: Focus on Biotechnology

Biochemistry, general - Short description currently not available.

Autoren/Hrsg.

Hofman, M.

Thonart, P.

Gembloux,

(Eds.), Belgium

Inhaltsverzeichnis

1;EDITORS PREFACE;6
2;TABLE OF CONTENTS;7
3;PART I UPSTREAM PROCESSES AND FERMENTATION;23
3.1;PRETREATMENT PROCESSES OF MOLASSES FOR THE UTILIZATION IN FERMENTATION PROCESSES;24
3.1.1;Abstract;24
3.1.2;1. Introduction;24
3.1.3;2. Materials and methods;25
3.1.4;3. Results and discussions;27
3.1.5;4. Conclusions;30
3.1.6;Acknowledgements;31
3.1.7;References;31
3.2;LACTIC ACID FERMENTATION OF HEMICELLULOSE LIQUORS AND THEIR ACTIVATED CARBON PRETREATMENTS ;32
3.2.1;Summary;32
3.2.2;1. Introduction;32
3.2.3;2. Materials and methods;33
3.2.4;3. Results and discussion;34
3.2.5;5. References;40
3.3;ENZYMIC SOLUBILISATION OF PROTEINS FROM TROPICAL TUNA USING ALCALASE AND SOME BIOLOGICAL PROPERTIES OF THE HYDROLYSATES;42
3.3.1;Summary;42
3.3.2;1. Introduction;42
3.3.3;2. Materials and methods;44
3.3.4;3. Results and discussion;47
3.3.5;4. Conclusion;52
3.4;INFLUENCE OF THE EXPERIMENTAL CONDITIONS ON THE HYDROLYSIS PROCESS IN FISH HYDROLYSATES.;54
3.4.1;Summary;54
3.4.2;1. Introduction;54
3.4.3;2. Materials and methods;55
3.4.4;3. Results and discussion;57
3.4.5;4. Conclusion;60
3.4.6;References;61
4;PART II PROCESS MODELLING;62
4.1;MATHEMATICAL MODELLING OF MICROBIAL PROCESSES – MOTIVATION AND MEANS;63
4.1.1;Abstract;63
4.1.2;1. Introduction;63
4.1.3;2. Motivation;64
4.1.4;3. Means - General modelling frameworks;66
4.1.5;4. Selected applications;72
4.1.6;5. Future prospects;74
4.1.7;References;75
4.2;MACROSCOPIC MODELLING OF BIOPROCESSES WITH A VIEW TO ENGINEERING APPLICATIONS;78
4.2.1;Abstract;78
4.2.2;1. Introduction;78
4.2.3;2. Macroscopic reaction network and associated mass balances;81
4.2.4;3. Kinetic model structure;85
4.2.5;4. Parameter identification;88
4.2.6;References;109
4.3;A MODEL DISCRIMINATION APPROACH FOR DATA ANALYSIS AND EXPERIMENTAL DESIGN;111
4.3.1;1. Introduction;111
4.3.2;2. Theoretical concept;113
4.3.3;3. Material and methods;116
4.3.4;4. Results and discussion;117
4.3.5;5. Conclusions;126
4.3.6;References;127
4.4;MODEL BASED SEQUENTIAL EXPERIMENTAL DESIGN FOR BIOPROCESS OPTIMISATION - AN OVERVIEW;129
4.4.1;Summary;129
4.4.2;1. Bioprocess modelling for experimental design procedures;130
4.4.3;2. Direct experimental design method;133
4.4.4;3. Indirect experimental design method;134
4.4.5;4. optimal experimental design method;137
4.4.6;5. Experimental Example;138
4.4.7;References;140
4.5;METABOLIC FLUX MODELLING AS A TOOL TO ANALYSE THE BEHAVIOR OF A GENETICALLY MODIFIED STRAIN OF SACCHAROMYCES CEREVISIAE;142
4.5.1;Abstract;142
4.5.2;1. Introduction;142
4.5.3;2. Materials and methods;144
4.5.4;3. Results and discussion;147
4.5.5;4. Conclusion and perspectives;153
4.5.6;References;154
4.6;METABOLIC INVESTIGATION OF AN ANAEROBIC CELLULOLYTIC;156
4.6.1;Abstract;156
4.6.2;1. Introduction;156
4.6.3;2. Material and method;157
4.6.4;3. Results and discussion;160
4.6.5;4. Conclusions and perspectives;164
4.6.6;Acknowledgement;165
4.6.7;References;165
5;PART III INTEGRATED PROCESSES;167
5.1;CROSSFLOW ULTRAFILTRATION OF BACILLUS LICHENIFORMIS FERMENTATION MEDIUM TO SEPARATE PROTEASE ENZYMES;168
5.1.1;Abstract;168
5.1.2;1. Introduction;168
5.1.3;2. Materials and methods;170
5.1.4;3. Results and Discussion;171
5.1.5;4. Conclusions;174
5.1.6;Acknowledgements;175
5.1.7;References;175
6;PART IV MONITORING AND CONTROL;177
6.1;EVALUATING DURING FERMENTATION USING MANY METHODS SIMULTANEOUSLY;178
6.1.1;Abstract;178
6.1.2;1. Introduction;178
6.1.3;2. Materials and methods;180
6.1.4;3. Results and discussion;188
6.1.5;4. Conclusion;194
6.1.6;Acknowledgements;194
6.1.7;References;195
6.1.8;Nomenclature;195
6.2;RESPIRATION QUOTIENT: ESTIMATION DURING BATCH CULTIVATION IN BICARBONATE BUFFERED MEDIA;197
6.2.1;Abstract;197
6.2.2;1. Introduction;197
6.2.3;2. Gas concentrations in batch- wise cell cultures;198
6.2.4;3. Software sensor design;202
6.2.5;5. Concluding remarks;209
6.2.6;References;210
6.3;FERMENTATION PHASE DETECTION USING FUZZY CLUSTERING TECHNIQUES AND NEURAL NETWORKS FOR IMPROVED CONTROL;211
6.3.1;1. Introduction;211
6.3.2;2. Fermentation phase detection;212
6.3.3;3. Conclusion;219
6.3.4;References;219
6.4;SIMULATION, DESIGN AND MODEL BASED PREDICTIVE CONTROL OF PHOTOBIOREACTORS;221
6.4.1;Abstract;221
6.4.2;1. Introduction;221
6.4.3;2. Modelling photobioreactors;222
6.4.4;3. Results and discussions;228
6.4.5;4. Conclusions and perspectives;230
6.4.6;References;232
7;PARTY V REACTOR ENGINEERING;233
7.1;BIOREACTORS FOR SPACE : BIOTECHNOLOGY OF THE NEXT CENTURY;234
7.1.1;Summary;234
7.1.2;1. Introduction;234
7.1.3;2. Space bioreactors : instrument;235
7.1.4;3. Space bioreactor SBRI: performances in flight;239
7.1.5;4. Conclusions and perspectives;243
7.1.6;Acknowledgements;244
7.1.7;References;244
8;PART VI IMMOBILISATION AND PERMEABILISATION;245
8.1;STATE OF THE ART DEVELOPMENTS IN IMMOBILISED YEAST TECHNOLOGY FOR BREWING;246
8.1.1;1. Process requirements for high turnover rates in brewery fermentations;246
8.1.2;2. Matrix design for application in the brewing process;248
8.1.3;3. Reactor design for application in the brewing process;249
8.1.4;4. Reactor configuration for continuous immobilised yeast fermentation systems;250
8.1.5;5. Flavour development and control in immobilised yeast systems;252
8.1.6;6. Technological potential of options for immobilised yeast application in the brewing industry;253
8.1.7;7. Concluding remarks;265
8.1.8;References;265
8.2;IMMOBILIZED YEAST BIOREACTOR SYSTEMS FOR BREWING – RECENT ACHIEVEMENTS;268
8.2.1;1. Immobilised cell systems in biotechnology;268
8.2.2;2. Applications of immobilised yeast systems in brewing;269
8.2.3;3. Alginate-gas-lift bioreactor system;274
8.2.4;4. Conclusion;280
8.2.5;References;280
8.3;NEW MATRICES AND BIOENCAPSULATION PROCESSES;284
8.3.1;Summary;284
8.3.2;1. Introduction;284
8.3.3;2. Techniques for bead production;286
8.3.4;3. Materials for encapsulation;292
8.3.5;4. LentiKats®;293
8.3.6;5. Conclusions;297
8.3.7;References;297
9;PART VII DOWNSTREAM PROCESSING;299
9.1;INDUSTRIAL DOWNSTREAM PROCESSING;300
9.1.1;1. Introduction.;300
9.1.2;2. General aspects connected to downstream processing.;300
9.1.3;3. Pharmaceutical production.;303
9.1.4;4. Enzyme production.;307
9.1.5;5. Summary.;312
9.1.6;References.;312
9.2;SEPARATION OF -LACTALBUMIN AND -LACTOGLOBULIN BY PREPARATIVE CHROMATOGRAPHY USING SIMULATED MOVING BEDS;314
9.2.1;Abstract;314
9.2.2;1. Introduction;314
9.2.3;2. Basic concepts of processes of separation with simulated moving beds;316
9.2.4;3. Mathematical formulation;317
9.2.5;4. Adsorption isotherms;319
9.2.6;5. Results and discussion;319
9.2.7;References;325
9.2.8;Appendix;326
9.3;HIGH-SPEED PECTIC ENZYME FRACTIONATION BY IMMOBILISED METAL ION AFFINITY MEMBRANES;327
9.3.1;Abstract;327
9.3.2;1. Introduction;327
9.3.3;2. Materials and methods;329
9.3.4;3. Results and discussion;332
9.3.5;4. Conclusions;336
9.3.6;Acknowledgements;336
9.3.7;References;336
10;PART VIII ECONOMIC FINALITIES;338
10.1;ECONOMIC BENEFITS OF THE APPLICATION OF BIOTECHNOLOGY - EXAMPLES;339
10.1.1;Summary;339
10.1.2;Overview;339
10.1.3;1. Production of 7-aminocephalosporanic acid;340
10.1.4;2. Stonewashing of jeans;340
10.1.5;3. Production of riboflavin;341
10.1.6;4. Biopulping;342
10.1.7;5. Bleach cleanup;342
10.1.8;6. Conclusions;346
10.1.9;References;346
10.2;ENZYME STABILITY AND STABILISATION : APPLICATIONS AND CASE STUDIES.;347
10.2.1;Summary;347
10.2.2;1. Introduction;347
10.2.3;2. Materials and methods;349
10.2.4;3. Results;350
10.2.5;4. Discussion and conclusions;360
10.2.6;Acknowledgement;361
10.2.7;References;361
10.3;IMPROVEMENTS OF ENZYME STABILITY AND SPECIFICITY BY GENETIC ENGINEERING;363
10.3.1;1. Introduction;363
10.3.2;2. Results;363
10.3.3;3. Conclusion;368
10.3.4;Acknowledgement;368
10.3.5;References;368
10.4;AN APPROACH TO DESICCATION-TOLERANT BACTERIA IN STARTER CULTURE PRODUCTION;369
10.4.1;1. Introduction;369
10.4.2;2. Selection of desiccation-tolerant bacteria;370
10.4.3;3. Targets of desiccation damages and the proposed mechanisms responsible for dessication tolerance;371
10.4.4;4. Factors influencing survival;379
10.4.5;5. Conclusions;381
10.4.6;Acknowledgements;382
10.4.7;References;382
10.5;BIOTECHNOLOGICAL RESEARCH AND THE DAIRY INDUSTRY: A Functional Interaction;385
10.5.1;Abstract;385
10.5.2;1. Introduction;385
10.5.3;2. Classification of lactic acid bacteria;386
10.5.4;3. Lactic acid bacteria as starter cultures;388
10.5.5;4. Improved starter strains – case studies;391
10.5.6;5. Outlook and Conclusions;395
10.5.7;Acknowledgements;396
10.5.8;References;396
10.6;IMMOBILISED CELL TECHNOLOGY IN WINERY AND FRUIT WINE PRODUCTION.;399
10.6.1;Summary;399
10.6.2;1. Introduction;399
10.6.3;2. Immobilised cell concept;400
10.6.4;3. Possible applications in winery and fruit wine production;401
10.6.5;4. Conclusion;405
10.6.6;References;405
10.7;A NEW POLYSACCHARIDE DERIVED FROM PLANT RHIZOSPHERE: PRODUCTION, PURIFICATION AND PHYSICO-CHEMICAL PROPERTIES;408
10.7.1;Summary;408
10.7.2;1. Introduction;408
10.7.3;2. Materials and methods;409
10.7.4;3. Results and discussion;410
10.7.5;4. Conclusion;412
10.7.6;Acknowledgements;413
10.7.7;References;413
10.8;INITIATION, GROWTH AND IMMOBILISATION OF CELL CULTURES OF TAXUS SPP. FOR PACLITAXEL PRODUCTION;414
10.8.1;Summary;414
10.8.2;1. Introduction;414
10.8.3;2. Materials and methods;417
10.8.4;3. Results and discussion;421
10.8.5;4. Conclusions;429
10.8.6;Acknowledgement;429
10.8.7;References;430
10.9;EFFECTIVE BIOFUEL PRODUCTION BY AN INTELLIGENT BIOREACTOR;434
10.9.1;Abstract;434
10.9.2;1. Introduction;434
10.9.3;2. Key technologies for biofuel production;435
10.9.4;3. Outline of ongoing research;437
10.9.5;4. Conclusion;439
10.9.6;References;439
11;PART IX PATENTS AND LICENSES;441
11.1;TRANSLATING EUROPEAN BIOTECH INTO US PATENTS DO’S, DON’TS, & COSTS;442
11.1.1;Introduction;442
11.1.2;1. Five important patent differences between Europe and the US;442
11.1.3;2. Basic patent game theory;443
11.1.4;3. Invention germination;444
11.1.5;4. Invention selection;445
11.1.6;5. Points of decision;447
11.1.7;6. Its just business;449
11.1.8;7. Filing a patent application;452
11.1.9;8. The prior of prior art;455
11.1.10;9. Making U.S. filings/incurring;456
11.1.11;10. Timing;462
11.1.12;11. Patent Position in Action;465
11.1.13;12. Digging for patent dirt;468
11.1.14;Conclusion;469
12;INDEX;470