Hoffmann-Ostenhof / Breitenbach / Koller Affinity Chromatography

Biospecific Sorption - The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry
1. Auflage 2016
ISBN: 978-1-4831-8836-2
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 390 Seiten, Web PDF

Affinity Chromatography is a collection of papers that presents the advancement in various areas of affinity chromatography. The title particularly covers the development in affinity chromatography in the context of biochemistry and immunochemistry. The text first details gel matrices, coupling methods, and charge transfer chromatography. Next, the selection tackles the affinity chromatography of enzymes and hydrophobic interaction chromatography. The text also discusses matrix-bound antigens and antibodies, along with matrix-bound lectins and affinity chromatography of cells. Part VI deals with affinity chromatography of hormone receptors, while part vii talks about affinity techniques in the isolation Of DNA, RNA, and polysomes. The book will be of great use to students, researchers, and practitioners of biochemistry.

Autoren/Hrsg.

Hoffmann-Ostenhof, O.

Breitenbach, M.

Koller, F.

Inhaltsverzeichnis

1;Front Cover;1
2;Affinity Chromatography;4
3;Copyright Page;5
4;Table of Contents;6
5;LIST OF PARTICIPANTS;12
6;INTRODUCTORY REMARKS;18
7;PART I: GEL MATRICES, COUPLING METHODS AND CHARGE TRANSFER CHROMATOGRAPHY;20
7.1;CHAPTER 1. GEL MATRICES, COUPLING METHODS AND CHARGE TRANSFER CHROMATOGRAPHY;22
7.1.1;CONTENTS;22
7.1.2;1. INTRODUCTION;22
7.1.3;2. MATRICES, COUPLING METHODS;23
7.1.4;3. CHARGE-TRANSFER CHROMATOGRAPHY;32
7.1.5;4. REFERENCES;38
8;PART II: AFFINITY CHROMATOGRAPHY OF ENZYMES;40
8.1;CHAPTER 2. AFFINITY CHROMATOGRAPHY OF ENZYMES;42
8.1.1;ABSTRACT;42
8.1.2;INTRODUCTION;42
8.1.3;SELECTION OF THE LIGAND;42
8.1.4;NUCLEOTIDE GROUP-SPECIFIC ADSORBENTS;43
8.1.5;TRIAZINE-LINKED DYES AS GROUP-SPECIFIC LIGANDS;48
8.1.6;ELECTROPHORETIC DESORPTION OF AFFINITY MATRICES;52
8.1.7;GENERAL CONCLUSIONS;52
8.1.8;ACKNOWLEDGEMENTS;52
8.1.9;REFERENCES;54
8.2;CHAPTER 3. AFFINITY CHROMATOGRAPHY ON IMMOBILISED COENZYMES;56
8.2.1;REFERENCES;68
8.3;CHAPTER 4. IMMOBILIZED ADENINE COENZYMES IN GENERAL LIGAND AFFINITY CHROMATOGRAPHY AND THEIR USE AS ACTIVE COENZYMES;72
8.3.1;A. GENERAL LIGAND AFFINITY CHROMATOGRAPHY;73
8.3.2;B. IMMOBILIZED ACTIVE COENZYME PREPARATIONS;79
8.3.3;REFERENCES;82
8.4;CHAPTER 5. DISSOCIATION CONSTANTS OF PHOSPHORYLASES TO OLIGO- AND POLYGLUCANS STUDIED BY THE AFFINITY ELECTROPHORESIS;84
8.4.1;METHODS;84
8.4.2;RESULTS AND DISCUSSION;86
8.4.3;REFERENCES;87
8.5;CHAPTER 6. AFFINITY CHROMATOGRAPHY AND CONFORMATIONAL ISOMERS OF dCMP-AMINOHYDROLASE;88
8.5.1;ABSTRACT;88
8.5.2;INTRODUCTION;88
8.5.3;MATERIALS AND METHODS;89
8.5.4;RESULTS AND DISCUSSION;89
8.5.5;REFERENCES;92
8.6;CHAPTER 7. OPTIMISATION OF CONDITIONS FOR THE AFFINITY CHROMATOGRAPHY OF HUMAN ENTEROKINASE;94
8.6.1;INTRODUCTION;94
8.6.2;MATERIALS AND METHODS;94
8.6.3;RESULTS AND DISCUSSION;94
8.6.4;REFERENCES;97
8.7;CHAPTER 8. AFFINITY CHROMATOGRAPHY OF BRAIN GLUTAMATE DECARBOXYLASE (EC 4.1.1.15) USING IMMOBILIZED PYRIDOXAL PHOSPHATE;98
8.7.1;REFERENCES;101
8.8;CHAPTER 9. AFFINITY CHROMATOGRAPHY OF TRYPTOPHAN SYNTHASE FROM E. COLI;102
8.8.1;ABSTRACT;102
8.8.2;INTRODUCTION;102
8.8.3;MATERIALS AND METHODS;102
8.8.4;RESULTS;103
8.8.5;DISCUSSION;105
8.8.6;REFERENCES;106
8.9;CHAPTER 10. AFFINITY CHROMATOGRAPHY OF THE PYRUVATE DEHYDROGENASE COMPLEX USING TPP AS A LIGAND;108
8.9.1;INTRODUCTION;108
8.9.2;DISCUSSION;111
8.9.3;REFERENCES;111
8.10;CHAPTER 11. TEMPERATURE DEPENDENCE, ACTIVATION ENERGY AND ENTHALPY CHANGE OF THE BINDING PROCESS OF UDP-GALACTOSE 4'-EPIMERASE TO ITS IMMOBILIZED SUBSTRATE;112
8.10.1;REFERENCES;114
8.11;CHAPTER 12. AFFINITY CHROMATOGRAPHY OF CHYMOTRYPSIN ON SOYBEAN TRYPSIN INHIBITOR SEPHAROSE: APPLICATIONS IN GENETICS AND NUCLIDE LABELLING;116
8.11.1;ACKNOWLEDGEMENTS;119
8.11.2;REFERENCES;119
8.12;CHAPTER 13. REVERSIBLE IMMOBILIZATION OF VIBRIO CHOLERAE NEURAMINIDASE BY BIOSPECIFIC SORPTION;120
8.12.1;ABSTRACT;120
8.12.2;INTRODUCTION;120
8.12.3;MATERIALS AND METHODS;121
8.12.4;RESULTS AND DISCUSSION;121
8.12.5;REFERENCES;123
8.13;CHAPTER 14. AFFINITY CHROMATOGRAPHY OF CARBOHYDRATE BINDING PROTEINS. PREPARATION AND USE OF SOME POTENTIAL LIGANDS;124
8.13.1;ABSTRACT;124
8.13.2;METHODS;124
8.13.3;RESULTS;125
8.13.4;DISCUSSION;127
8.13.5;REFERENCES;127
8.14;CHAPTER 15. DIRECT COUPLING OF REDUCING

OLIGOSACCHARIDES TO

AMINOHEXYL-SEPHAROSE:

PURIFICATION OF a,a-TREHALASE FROM

ARTEMIA SA LINA;128
8.14.1;ABSTRACT;128
8.14.2;INTRODUCTION;128
8.14.3;METHODS;128
8.14.4;RESULTS;129
8.14.5;DISCUSSION;131
8.14.6;REFERENCES;131
8.15;CHAPTER 16. PURIFICATION OF PECTINESTERASE FROM ASPERGILLUS NIGER BY CHROMATOGRAPHY ON AN AMIDE DERIVATIVE OF THE CROSS-LINKED PECTATE;132
8.15.1;MATERIALS AND METHOD;132
8.15.2;RESULTS;133
8.15.3;DISCUSSION;134
8.15.4;CONCLUSION;135
8.15.5;REFERENCES;135
8.16;CHAPTER 17. A CHYMOTRYPSIN INHIBITOR FROM ADULT CULEX PIPIENS ISOLATED BY AFFINITY CHROMATOGRAPHY;136
8.16.1;Isolation of the inhibitor;136
8.16.2;Some properties of the inhibitor;138
8.16.3;References;139
8.17;CHAPTER 18. SEPARATION OF PROTEINASES AND IN PARTICULAR OF CLOSTRIPAIN AND COLLAGENASE BY AFFINITY CHROMATOGRAPHY;140
8.17.1;ABSTRACT;140
8.17.2;EXPERIMENTAL AND RESULTS;140
8.17.3;REFERENCES;143
8.18;CHAPTER 19. SYNTHESIS OF IMIDAZOLE CONTAINING MATRICES (POLYHYDROXYETHYLMETHACRYLATES, POLYSACCHARIDES) AND ITS APPLICATION IN AFFINITY CHROMATOGRAPHY;146
8.18.1;ABSTRACT;146
8.18.2;INTRODUCTION;146
8.18.3;SYNTHESIS OF IMIDAZOLE CONTAINING MATRICES;146
8.18.4;ADSORPTION OF HEMOGLOBIN;148
8.18.5;APPLICATION OF IMIDAZOLE CONTAINING MATRICES;148
8.18.6;REFERENCES;149
8.19;CHAPTER 20. PURIFICATION OF NEUTRAL PROTEASES FROM LEUCOCYTES ON PROTAMINE-SEPHAROSE;150
8.19.1;MATERIALS AND METHODS;150
8.19.2;RESULTS;151
8.19.3;DISCUSSION;153
8.19.4;REFERENCES;153
9;PART III: HYDROPHOBIC INTERACTION CHROMATOGRAPHY;156
9.1;CHAPTER 21. HOMOLOGOUS SERIES OF HYDROCARBON-COATED AGAROSE IN HYDROPHOBIC CHROMATOGRAPHY;158
9.1.1;FROM BIOSPECIFIC AFFINITY CHROMATOGRAPHY TO HYDROPHOBIC CHROMATOGRAPHY;158
9.1.2;USEFUL FEATURES AND POTENTIAL OF THIS APPROACH;159
9.1.3;STRUCTURE OF HYDROCARBON-COATED AGARQSES;162
9.1.4;ON THE MECHANISM OF ACTION OF HYDROCARBON-COATED AGAROSES;167
9.1.5;CONCLUDING REMARKS;175
9.1.6;ACKNOWLEDGEMENT;175
9.1.7;REFERENCES;175
9.2;CHAPTER 22. ADSORPTION OF PROTEINS AT HIGH SALT CONCENTRATIONS ON HYDROPHOBICALLY INTERACTING MATRICES;178
9.2.1;INTRODUCTION;178
9.2.2;HYDROPHOBIC INTERACTION;179
9.2.3;HYDROPHOBIC INTERACTION CHROMATOGRAPHY;181
9.2.4;APPLICATIONS;185
9.2.5;CONCLUSION;187
9.2.6;REFERENCES;188
9.3;CHAPTER 23. THE BINDING OF MYOGLOBIN AND HEMOGLOBIN TO HYDROPHOBICALLY SUBSTITUTED SEPHAROSES;192
9.3.1;INTRODUCTION;192
9.3.2;MATERIALS AND METHODS;192
9.3.3;RESULTS AND DISCUSSION;193
9.3.4;REFERENCES;196
9.4;CHAPTER 24. ON THE PURIFICATION OF BACTERIAL TYROSINE DECARBOXYLASE;198
9.4.1;INTRODUCTION;198
9.4.2;MATERIALS AND METHODS;198
9.4.3;RESULTS;201
9.4.4;REFERENCES;201
9.5;CHAPTER 25. IMPROVED SYNTHESIS OF A FATTY ACID-AGAROSE COMPLEX FOR AFFINITY CHROMATOGRAPHY OF SERUM ALBUMIN;202
9.5.1;INTRODUCTION;202
9.5.2;METHODS;202
9.5.3;RESULTS;204
9.5.4;REFERENCES;204
10;PART IV: MATRIX-BOUND ANTIGENS AND ANTIBODIES;206
10.1;CHAPTER 26. MATRIX-BOUND ANTIGENS AND ANTIBODIES;208
10.1.1;INTRODUCTION;208
10.1.2;GENERAL FEATURES OF IMMUNOADSORBENTS;208
10.1.3;THE MATRIX;210
10.1.4;COUPLING OF IMMUNOLIGANDS;212
10.1.5;METHODS TO ATTENUATE ANTIGEN AND ANTIBODY AFFINITY;216
10.1.6;DESORPTION OF IMMUNOADSORBENTS;216
10.1.7;REFERENCES;219
10.2;CHAPTER 27. COMPARISON OF SEPHAROSE AND CELLULOSE AS A SUPPORT FOR ANTIBODY AND ANTIGEN;224
10.2.1;ABSTRACT;224
10.2.2;INTRODUCTION;224
10.2.3;MATERIALS & METHODS;224
10.2.4;RESULTS;225
10.2.5;DISCUSSION;225
10.2.6;REFERENCES;227
10.3;CHAPTER 28. APPROACH TO THE SEQUENCE ANALYSIS OF PROTEINS WITH HLA ACTIVITY BY AFFINITY CHROMATOGRAPHY;228
10.3.1;ABSTRACT;228
10.3.2;REFERENCES;230
10.4;CHAPTER 29. BIO- AND IMMUNOSPECIFIC AFFINITY CHROMATOGRAPHY OF PLASMA MEMBRANE ANTIGENS FROM HUMAN KIDNEY AND RENAL CELL CARCINOMA;232
10.4.1;INTRODUCTION;232
10.4.2;METHODS;232
10.4.3;RESULTS;233
10.4.4;DISCUSSION;235
10.4.5;REFERENCES;235
10.5;CHAPTER 30. THE PURIFICATION OF MELANOMA ANTIGEN FROM HUMAN URINE;236
10.5.1;SUMMARY;236
10.5.2;INTRODUCTION;236
10.5.3;METHODS AND MATERIALS;236
10.5.4;RESULTS;237
10.5.5;DISCUSSION;237
10.5.6;ACKNOWLEDGEMENTS;239
10.5.7;REFERENCES;239
10.6;CHAPTER 31. BENZYLPENICILLOYL (BPO)-SPECIFIC AFFINITY AND SUBSEQUENT ION EXCHANGE CHROMATOGRAPHY: CHARACTERIZATION OF ANTIBODY SUBFRACTIONS;240
10.6.1;INTRODUCTION;240
10.6.2;MATERIAL AND METHODS;240
10.6.3;RESULTS AND DISCUSSION;241
10.6.4;SUMMARY;243
10.6.5;ACKNOWLEDGMENT;244
10.6.6;REFERENCES;244
10.7;CHAPTER 32. IMMUNOSORBENT SEPARATION OF IgG AND IgM FOR THE RADIOIMMUNOASSAY OF SPECIFIC ANTIBODIES;246
10.7.1;INTRODUCTION;246
10.7.2;MATERIALS AND METHODS;246
10.7.3;RESULTS AND DISCUSSION;247
10.7.4;REFERENCES;248
10.8;CHAPTER 33. ELUTION BY GLUCOSE OF BOVINE IMMUNOGLOBULINS ADSORBED ON CROSS-LINKED DEXTRAN;250
10.8.1;Materials and Methods;250
10.8.2;References;253
10.8.3;Results and Discussion;251
10.8.4;References;253
10.9;CHAPTER 34. PURIFICATION OF ENZYME-LABELLED CONJUGATE BY AFFINITY CHROMATOGRAPHY;254
10.9.1;ABSTRACT;254
10.9.2;INTRODUCTION;254
10.9.3;METHODS;255
10.9.4;REFERENCES;258
10.10;CHAPTER 35. KILLED STAPHYLOCOCCAL AUREUS CELLS AS SUPPORT FOR ANTIBODY MEDIATED AFFINITY CHROMATOGRAPHY;260
10.10.1;ABSTRACT;260
10.10.2;INTRODUCTION;260
10.10.3;MATERIALS AND METHODS;260
10.10.4;RESULTS;261
10.10.5;DISCUSSION;263
10.10.6;REFERENCES;263
10.11;CHAPTER 36. THE USE OF AFFINITY CHROMATOGRAPHY FOR PURIFICATION OF ENZYME - ANTIBODY CONJUGATES;264
10.11.1;ABSTRACT;264
10.11.2;INTRODUCTION;264
10.11.3;MATERIALS AND METHODS;264
10.11.4;RESULTS;265
10.11.5;DISCUSSION;266
10.11.6;REFERENCES;267
10.12;CHAPTER 37. PURIFICATION OF DD-CARBOXYPEPTIDASES FROM STREPTOMYCES STRAINS R61 AND K15 BY ANTIGEN-ANTIBODY AFFINITY CHROMATOGRAPHY;268
10.12.1;ABSTRACT;268
10.12.2;INTRODUCTION;268
10.12.3;PURIFICATION OF THE IgG FRACTION;269
10.12.4;ENZYME PURIFICATION;270
10.12.5;CONCLUSION;271
10.12.6;REFERENCES;271
10.12.7;ACKNOWLEDGEMENTS;272
10.13;CHAPTER 38. SELECTIVE REMOVAL OF FAST DISSOCIATING ANTIBODIES FROM A HIGH-AFFINITY THYROXINE ANTISERUM TO BE USED IN RADIO-IMMUNOASSAY;274
10.13.1;ABSTRACT;274
10.13.2;INTRODUCTION;274
10.13.3;PRINCIPLE;275
10.13.4;MATERIAL AND METHODS;276
10.13.5;RESULTS AND DISCUSSION;276
10.13.6;REFERENCES;277
10.14;CHAPTER 39. ADSORPTION AND ELUTION CHARACTERISTICS OF P. AERUGINOSA TYPE 2 ANTIBODY IN AN AFFINITY CHROMATOGRAPHIC SYSTEM;278
10.14.1;REFERENCES;281
10.15;CHAPTER 40. BIOSPECIFIC CHROMATOGRAPHY ON NEW DERIVATIVES OF POROUS SILICA BEADS. COUPLING OF GANGLIOSIDE GM1 OR ANTICHOLERAGEN ANTIBODY FOR PURIFICATION OF CHOLERA TOXIN;282
10.15.1;SUMMARY;282
10.15.2;INTRODUCTION;282
10.15.3;MATERIALS AND METHODS;283
10.15.4;RESULTS AND DISCUSSION;284
10.15.5;ACKNOWLEDGEMENTS;286
10.15.6;REFERENCES;286
11;PART V: MATRIX-BOUND LECTINS AND AFFINITY CHROMATOGRAPHY OF CELLS;288
11.1;CHAPTER 41. FRACTIONATION OF LYMPHOCYTES ON INSOLUBILIZED HELIX POMA TIA A HEMAGGLUTININ AND WHEAT GERM AGGLUTININ;290
11.1.1;INTRODUCTION;290
11.1.2;MATERIALS AND METHODS;290
11.1.3;RESULTS;293
11.1.4;CONCLUSIONS;301
11.1.5;ACKNOWLEDGEMENTS;302
11.1.6;REFERENCES;302
11.2;CHAPTER 42. INVESTIGATION OF THE OLIGOSACCHARIDE HETEROGENEITY OF HUMAN ENTEROKINASE BY AFFINITY CHROMATOGRAPHY ON IMMOBILISED LECTINS;304
11.2.1;INTRODUCTION;304
11.2.2;MATERIALS AND METHODS;304
11.2.3;REFERENCES;307
11.3;CHAPTER 43. IN GLYCOPROTEINS;308
11.3.1;INTRODUCTION;308
11.3.2;EXPERIMENTAL;309
11.3.3;RESULTS AND DISCUSSION;309
11.3.4;REFERENCES;311
11.4;CHAPTER 44. BINDING SPECIFICITY AND PURIFICATION OF MEDIC AGO SATIVA LECTIN;312
11.4.1;INTRODUCTION;312
11.4.2;METHODS;312
11.4.3;RESULTS AND DISCUSSION;313
11.4.4;REFERENCES;315
11.5;CHAPTER 45. CROSS-LINKED ERYTHROCYTE MEMBRANE COLUMNS AS A TOOL FOR AFFINITY CHROMATOGRAPHY OF LECTINS;316
11.5.1;INTRODUCTION;316
11.5.2;METHODS;316
11.5.3;RESULTS;317
11.5.4;DISCUSSION;318
11.5.5;REFERENCES;319
11.6;CHAPTER 46. AFFINITY CHROMATOGRAPHY OF MEMBRANE VESICLES;320
11.6.1;ABSTRACT;320
11.6.2;INTRODUCTION;320
11.6.3;METHODS;320
11.6.4;RESULTS;320
11.6.5;DISCUSSION;322
11.6.6;REFERENCES;323
11.7;CHAPTER 47. ON THE MECHANISM OF ADSORPTION OF ERYTHROCYTES TO HYDROCARBON-COATED AGAROSES;324
11.7.1;ABSTRACT;324
11.7.2;INTRODUCTION;324
11.7.3;MATERIALS AND METHODS;325
11.7.4;RESULTS AND DISCUSSION;325
11.7.5;ACKNOWLEDGEMENT;327
11.7.6;REFERENCES;328
11.8;CHAPTER 48. ENRICHMENT OF AVIAN ANTIGEN BINDING CELLS (ABC) BY AFFINITY CHROMATOGRAPHY;330
11.8.1;SUMMARY;330
11.8.2;INTRODUCTION;330
11.8.3;MATERIAL AND METHODS;331
11.8.4;RESULTS AND DISCUSSION;331
11.8.5;ACKNOWLEDGEMENTS;333
11.8.6;REFERENCES;333
12;PART VI: AFFINITY CHROMATOGRAPHY OF HORMONE RECEPTORS;334
12.1;CHAPTER 49. ISOLATION OF HORMONE RECEPTORS BY AFFINITY CHROMATOGRAPHY;336
12.1.1;INSULIN RECEPTOR;336
12.1.2;ESTROGEN RECEPTORS;339
12.1.3;REFERENCES;343
12.2;CHAPTER 50. SOLUBLE BIOSPECIFIC MACROMOLECULE FOR PURIFICATION OF ESTROGEN RECEPTOR;346
12.2.1;References;349
13;PART VII: AFFINITY TECHNIQUES IN THE ISOLATION OF DNA, RNA AND POLYSOMES;352
13.1;CHAPTER 51. AFFINITY TECHNIQUES IN THE ISOLATION OF SPECIFIC POLYSOMES AND mRNA: PURIFICATION OF RAT ALBUMIN mRNA;354
13.1.1;ABSTRACT;354
13.1.2;INTRODUCTION;354
13.1.3;PREPARATION OF SPECIFIC ANTIBODIES;356
13.1.4;CHARACTERIZATION OF ANTIBODY BINDING;356
13.1.5;POLYSOME IMMUNOPRECIPITATION;358
13.1.6;CHARACTERIZATION OF THE POLYSOME IMMUNOPRECIPITATE;360
13.1.7;PURIFICATION OF mRNA BY AFFINITY CHROMATOGRAPHY;362
13.1.8;CHARACTERIZATION OF THE PURIFIED mRNA;364
13.1.9;DISCUSSION;366
13.1.10;ACKNOWLEDGEMENT;367
13.1.11;REFERENCES;367
13.2;CHAPTER 52. SYNTHESIS OF A NOVEL MATERIAL FOR AFFINITY CHROMATOGRAPHY OF BIOPOLYMERS AND ITS APPLICATION FOR FRACTIONATION OF NUCLEIC ACIDS;370
13.2.1;REFERENCES;373
13.3;CHAPTER 53. PREPARATION OF AFFINITY-ADSORBENTS CONTAINING NUCLEIC ACIDS IMMOBILIZED ONTO CARBOXYMETHYL-CELLULOSE;374
13.3.1;INTRODUCTION;374
13.3.2;EXPERIMENTAL PROCEDURES;374
13.3.3;RESULTS AND DISCUSSION;375
13.3.4;REFERENCES;376
13.4;CHAPTER 54. PURIFICATION OF ANTIBODIES SPECIFIC FOR PHOTOPRODUCTS OTHER THAN PYRIMIDINE DIMERS IN ULTRAVIOLET-LIGHT IRRADIATED DNA BY AFFINITY CHROMATOGRAPHY;378
13.4.1;Abstract;378
13.4.2;Introduction;378
13.4.3;Materials and Methods;379
13.4.4;Discussion;381
14;SUBJECT INDEX;382