Programmed Morphogenesis

Methods and Protocols
1. Auflage 2021
ISBN: 978-1-0716-1176-0
Verlag: Springer US

Buch, Englisch, Band 2258, 286 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 569 g

Reihe: Methods in Molecular Biology

This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Hislop, Joshua

Ebrahimkhani, Mo R.

Fachgebiete

• Naturwissenschaften Biowissenschaften Entwicklungsbiologie
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie

Inhaltsverzeichnis

TASBE Image Analytics: A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy.- Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities.- A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut.- Reconstitution of Morphogen Signaling Gradients in Cultured Cells.- Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands.- Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives.- Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model.- Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells.- Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells.- Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos.- A Synergistic Engineering Approach toBuild Human Brain Spheroids.- Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids.- Methods for Controlled Induction of Singular Rosette Cytoarchitecture within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies.- 3D Self-Organized Human Blood-Brain Barrier in a Microfluidic Chip.- Modeling the Complexity of the Metastatic Niche Ex Vivo.- Fabrication Method of a High-Density Co-Culture Tumor-Stroma Platform to Study Cancer Progression.- A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization.- High Throughput Production of Platelet-Like Particles.