Henry / Furtado Cereal Genomics

Methods and Protocols
2014
ISBN: 978-1-62703-715-0
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1099, 300 Seiten, eBook

Reihe: Methods in Molecular Biology

In Cereal Genomics: Methods and Protocols , expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Henry, Robert J.

Furtado, Agnelo

Inhaltsverzeichnis

DNA Extraction from Vegetative Tissue for Next Generation Sequencing  .- DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) .- RNA Extraction from Cereal Vegetative Tissue.- RNA Extraction from Developing or Mature Wheat Seeds.- cDNA Library Preparation.- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants.- The Polymerase Chain Reaction (PCR) - General Methods.- Mutation and Mutation Screening.- The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression.- Cloning of DNA Fragments: Ligation Reactions in Agarose Gel.- Rapid Cloning of Genes and Promoters for Functional Analyses.- Genome Walking.- Functional Analysis by Protein Biochemistry.- Genomic Southern Blot analysis.- Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs.- Protein Blotting-Protocol for Beginners.- Genetic Transformation of Wheat via Particle Bombardment.- Sorghum Genetic Transformation by Particle Bombardment.- Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery.- A Protocol for High-throughput Agrobacterium-mediated Barley Transformation.- Agrobacterium-mediated Transformation- Rice Transformation.- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos.- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops.- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.