Hawley Flow Cytometry Protocols

4th Auflage 2018
ISBN: 978-1-4939-7346-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1678, 492 Seiten, eBook

Reihe: Methods in Molecular Biology

This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition  researchers and scientists who are interested in continuing or expanding their knowledge of flow cytometry.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Hawley, Teresa S.

Hawley, Robert G.

Inhaltsverzeichnis

Flow Cytometry:- The Glass is Half Full.- High-Dimensional Modeling for Cytometry: Building Rock Solid Models using GemStone TM  and Verity Cen-se’ TM  t-family: Helvetica, sans-serif; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">High-Definition t-SNE Mapping.- Mass Cytometry Assays for Antigen-Specific T Cells using CyTOF.- RNA Flow Cytometry using the Branched DNA Technique.- Analysis of Individual Extracellular Vesicles by Flow Cytometry.- Quantitative Fluorescence Measurements with Multicolor Flow Cytometry.- High Throughput Flow Cytometry for Cell Surface Profiling.- Multiparameter Conventional Flow Cytometry.- Multiparameter Intracellular Cytokine Staining.- Multiparameter Analysis of Apoptosis by Flow Cytometry.- Multiparameter Cell Cycle Analysis.- Monitoring Cell Proliferation by Dye Dilution: Considerations for Probe Selection.- Immunophenotypic Identification of Early Myeloerythroid Development.- Flow Cytometry Assays in Primary Immunodeficiency Diseases.- Real-Time Deformability Cytometry: Label Free Functional Characterization of Cells.- Nuclear Cytometry: Analysis of the Patterns of DNA Synthesis and Transcription using Flow Cytometry, Confocal Microscopy, and RNA Sequencing.- Flow Cytometric FRET Analysis of Protein Interactions.- Overview of Fluorescence Lifetime Measurements in Flow Cytometry.- Overview of Lasers for Flow Cytometry.- Flow Cytometry: The Glass is Half Empty.