Hartmann / Bindereif / Schön Handbook of RNA Biochemistry

2. Completely Revised and Enlarged Auflage 2015
ISBN: 978-3-527-65055-2
Verlag: Wiley-VCH
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 1276 Seiten, E-Book

Ein umfassendes Kompendium für alle, die mit kleinen oder großen RNAs arbeiten. Bietet Hunderte von Protokollen, umfassende Erläuterungen und Tipps zur Fehlerbehebung, die in keinem anderen Nachschlagewerk zu finden sind.

Autoren/Hrsg.

Hartmann, Roland K.

Bindereif, Albrecht

Schön, Astrid

Westhof, Eric

Inhaltsverzeichnis

RNA SYNTHESIS
Enyzmatic synthesis, ligation and modification
Chemical synthesis
NEW: RNA detection and quantitation
 
STRUCTURE DETERMINATION
Sequence determination
NEW: Probing in vitro and in vivo
Footprinting techniques
Nucleotide analog interference mapping
Identification of interacting proteins and nucleic acids
Spectroscopic studies of RNA structure
Atomic structure determination
NEW: Functional assays
NEW: Mass spectrometry of RNA
NEW: Folding and unfolding of single RNA molecules
 
RNA GENOMICS & BIOINFORMATICS
Phylogenetic analysis
Structure prediction
Modeling of RNA structures
Molecular dynamics simulation
NEW: Searching and predicting small RNAs
NEW: RNA mining and RNomics in eukaryotes
NEW: Exon arrays
 
ANALYSIS OF RNA-PROTEIN INTERACTION
Isolation and purification of RNA-binding proteins
Sedimentation analysis of RNP complexes
Protein-RNA crosslinking
Immunoaffinity purification
NEW: TAP-tag affinity purification
Three-hybrid analysis to identify RNA-binding proteins
 
SELEX
In vitro selection of ribozymes and aptamers
In vivo SELEX
In vitro selection against small targets
NEW: Genomic SELEX
 
RNA INTERFERENCE
NEW: Chemical modification of siRNA
NEW: siRNA design and delivery
NEW: RNAi in mammalian cells
 
(partial contents)

Über Autor(innen)

Roland K. Hartmann is Professor of Pharmaceutical Chemistry at the Philipps-Universität Marburg (Germany). He studied Biochemistry at the Freie Universität Berlin where in 1988 he received the Ernst Reuter award for outstanding Ph.D. theses. His research interests include natural ribozymes, particularly ribonuclease P, interactions of RNAs with proteins and small ligands, aptamers, antisense and RNA interference.
 
Albrecht Bindereif is Professor of Biochemistry at the University of Giessen (Germany) since 1999. He studied Biochemistry in Tübingen and at the University of California, Berkeley. His postdoctoral work was done in Michael Green's laboratory at Harvard University. His research interests focus on the mechanism and regulation of mRNA splicing, both in the mammalian system and in trypanosomes.
 
Astrid Schön received her PhD from the Institute of Biochemistry at the University of Würzburg, and was a graduate fellow and postdoctoral associate with Dieter Söll at Yale University. She is currently a lecturer at the Institute of Biochemistry, University of Leipzig (Germany). Her research is focused on RNA metabolism and RNA-protein interactions, and the evolution of complex RNA enzymes.
 
Eric Westhof is Professor of Biophysics at the Université Louis Pasteur in Strasbourg (France) since 1988. He studied in Liège and Regensburg and was a Fulbright-Hays Research Fellow at the University of Wisconsin with M. Sundaralingam. His research is centered on the relationships between sequence, structure, evolution and functions of RNA molecules.