Hamdan / Righetti Proteomics Today

Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis,and Microarray Technology
1. Auflage 2005
ISBN: 978-0-471-70910-7
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 448 Seiten, E-Book

Reihe: Wiley-Interscience Series on Mass Spectrometry

The last few years have seen an unprecedented drive toward theapplication of proteomics to resolving challenging biomedical andbiochemical tasks. Separation techniques combined with modern massspectrometry are playing a central role in this drive. This bookdiscusses the increasingly important role of mass spectrometry inproteomic research, and emphasizes recent advances in the existingtechnology and describes the advantages and pitfalls as well.
* Provides a scientifically valid method for analyzing theapproximatey 500,000 proteins that are encoded in the humangenome
* Explains the hows and whys of using mass spectrometry inproteomic analysis
* Brings together the latest approaches combining separationtechniques and mass spectrometry and their application in proteomeanalysis
* Comments on future challenges and how they may be addressed
* Includes sections on troubleshooting

Autoren/Hrsg.

Hamdan, Mahmoud H.

Righetti, Pier G.

Inhaltsverzeichnis

PREFACE TO PART I.
ACKNOWLEDGMENT.
I: INTRODUCTION TO PART I.
1. INSTRUMENTATION AND DEVELOPMENTS.
1.1 Introduction.
1.2 Ionization Techniques for Macromolecules.
1.3 Examples on Analytical Solutions Based on FAB-MS.
1.4 Electrospray Ionization.
1.5 Matrix-Assisted Laser Desorption Ionization.
1.6 Ion Detection.
1.7 Types of Analyzers.
1.8 Hybrid Analyzers.
1.9 Tandem Mass Spectrometry.
1.10 Current MS Instrumentation in Proteome Analyses.
1.11 Current MS-Based Proteomics.
1.12 Recent Achievements and Future Challenges.
1.13 Concluding Remarks.
References.
2. PROTEOMICS IN CANCER RESEARCH.
2.1 Introduction.
2.2 Pancreatic Ductal Adenocarcinoma.
2.3 Proteomic Analysis of Human Breast Carcinoma.
2.4 Proteomic Profiling of Chemoresistant Cancer Cells.
2.5 Signal Pathway Profiling of Prostate Cancer.
2.6 Emerging Role of Functional and Activity-Based Proteomics inDisease Understanding.
2.7 Activity-Based Protein Profiling.
2.8 Probing Protein Functions Using Chromophore-Assisted LaserInactivation.
2.9 Role of Protein-Tyrosine Kinases.
2.10 Concluding Remarks and Future Prospects.
References.
3. CURRENT STRATEGIES FOR PROTEIN QUANTIFICATION.
3.1 Introduction.
3.2 Global Internal Standard Technology.
3.3 Differential In-Gel Electrophoresis.
3.4 Quantification of Modified Proteins.
3.5 Comments and Considerations.
3.6 Other Approaches.
3.7 Emerging Role of Microfluidic Devices.
3.8 Concluding Remarks.
References.
II: PROTEOMICS TODAY: SEPARATION SCIENCE AT WORK.
4. CONVENTIONAL ISOELECTRIC FOCUSING IN GEL MATRICES ANDCAPILLARIES AND IMMOBILIZED pH GRADIENTS.
4.1 Introduction.
4.2 Conventional Isoelectric Focusing in Amphoteric Buffers.
4.3 Immobilized pH Gradients.
4.4 Capillary Isoelectric Focusing.
4.5 Separation of Peptides and Proteins by CZE in IsoelectricBuffers.
4.6 Conclusions.
References.
5. SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELELECTROPHORESIS.
5.1 Introduction.
5.2 SDS-Protein Complexes: a Refinement of the Model.
5.3 Theoretical Background of Mr Measurement bySDS-PAGE.
5.4 Methodology.
5.5 Gel Casting and Buffer Systems.
5.6 Blotting Procedures.
5.7 Conclusions.
References.
6. TWO-DIMENSIONAL MAPS.
6.1 Introduction.
6.2 Some Basic Methodology Pertaining to 2D PAGE.
6.3 Prefractionation Tools in Proteome Analysis.
6.4 Multidimensional Chromatography Coupled to MS.
6.5 Protein Chips and Microarrays.
6.6 Nondenaturing Protein Maps.
6.7 Spot Matching in 2D Gels via Commercial Software.
6.8 Conclusions.
References.
INDEX.

Über Autor(innen)

MAHMOUD HAMDAN, PhD, is the head of Mass Spectrometry &Separation Technologies, GlaxoSmithKline, Verona, Italy.
PIER GIORGIO RIGHETTI, PhD, is Professor of Biochemistry anddirector of the Laboratory of Proteome Science at the University ofVerona, Italy.