Hall Regulators and Effectors of Small GTPases, Part G: Ras Family II

1. Auflage 2001
ISBN: 978-0-08-052256-2
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 333, 400 Seiten, Web PDF

Reihe: Methods in Enzymology

The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325, 329, and 332) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in the above-mentioned aspects of cell biology at the molecular level.

Autoren/Hrsg.

Hall, Alan

Inhaltsverzeichnis

1;Cover;1
2;Title Page;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors to Volume 333;10
6;Preface;16
7;Volumes in Series;18
8;Section I: Cytoplasmic and Nuclear Signaling Analyses;38
8.1;Chapter 1. Determining Involvement of Shc Proteins in Signaling Pathways;40
8.2;Chapter 2. Assaying Activity of Individual Protein Kinases in Crude Tissue or Cellular Extracts;53
8.3;Chapter 3. Recombinant Adenoviral Expression of Dominant- Negative Ras N17 Blocking Radiation- Induced Activation of Mitogen-Activated Protein Kinase Pathway;65
8.4;Chapter 4. Ras Activation of Phosphatidylinositol 3-Kinase and Akt;74
8.5;Chapter 5. Assays for Monitoring p70 S6 Kinase and RSK Activation;82
8.6;Chapter 6. Ras Activation of PAK Protein Kinases;92
8.7;Chapter 7. Ras Signaling to Transcription Activation: Analysis with GAL4 Fusion Proteins;98
8.8;Chapter 8. Ras Regulation of NF-.B and Apoptosis;110
8.9;Chapter 8. Ras Regulation of NF-.B and Apoptosis;125
8.10;Chapter 10. Ras, Metastasis, and Matrix Metalloproteinase 9;133
8.11;Chapter 11. Ras Regulation of Urokinase-Type Plasminogen Activator;142
8.12;Chapter 12. Ras Regulation of Cyclin D1 Promoter;153
8.13;Chapter 13. Ras Regulation of Cyclin-Dependent Immunoprecipitation Kinase Assays;164
8.14;Chapter 14. STAT Proteins: Signal Tranducers and Activators of Transcription;175
8.15;Chapter 15. Integrin Regulation of Receptor Tyrosine Kinase and G Protein-Coupled Receptor Signaling to Mitogen-Activated Protein Kinases;188
8.16;Chapter 16. R-Ras Regulation of Integrin Function;200
8.17;Chapter 17. Caveolin and Ras Function;209
9;Section II: Biological Analyses;222
9.1;Chapter 18. Analyses of M-Ras/R-Ras3 Signaling and Biology;224
9.2;Chapter 19. Analyses of TC21/R-Ras2 Signaling and Biological Activity;240
9.3;Chapter 20. Characterization of Rheb Functions Using Yeast and Mammalian Systems;254
9.4;Chapter 21. Ras Regulation of Skeletal Muscle Differentiation and Gene Expression;269
9.5;Chapter 22. Induction of Senescence by Oncogenic Ras;284
9.6;Chapter 23. Ras and Rho Protein Induction of Motility and Invasion in T47D Breast Adenocarcinoma Cells;293
9.7;Chapter 24. Ras Regulation of Vascular Endothelial Growth Factor and Angiogenesis;304
9.8;Chapter 25. Ras Regulation of Radioresistance in Cell Culture;321
9.9;Chapter 26. Paired Human Fibrosarcoma Cell Lines That Possess or Lack Endogenous Mutant N-ras Alleles as Experimental Model for Ras Signaling Pathways;327
9.10;Chapter 27. Orally Bioavailable Farnesyltransferase Inhibitors as Anticancer Agents in Transgenic and Xenograft Models;343
9.11;Chapter 28. Animal Models for Ras-Induced Metastasis;355
10;Section III: Regulation of Guanine Nucleotide Association;368
10.1;Chapter 29. Nonradioactive Determination of Ras–GTP Levels Using Activated Ras Interaction Assay;370
10.2;Chapter 30. Measurement of GTP-Bound Ras-Like GTPases by Activation-Specific Probes;380
10.3;Chapter 31. Immunocytochemical Assay for Ras Activity;385
11;Author Index;394
12;Subject Index;426