Guthrie Guide to Yeast Genetics and Molecular Cell Biology, Part B

1. Auflage 2002
ISBN: 978-0-08-049698-6
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 350, 623 Seiten, Web PDF

Reihe: Methods in Enzymology

This volume and its companion, Volume 351, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include basic techniques, making mutants, genomics, and proteomics.

Autoren/Hrsg.

Guthrie, Christine

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 350;10
4;Preface;16
5;Volume in Series;18
6;Section I: Basic Techniques;40
6.1;Chapter 1. Getting Started with Yeast;42
6.2;Chapter 2. How to Set up a Yeast Laboratory;81
6.3;Chapter 3. Constructing Yeast Libraries;111
6.4;Chapter 4. Transformation of Yeast by Lithium Acetate/Single-Stranded Carrier DNA/Polyethylene Glycol Method;126
6.5;Chapter 5. Genetic Transformation of Saccharomyces cerevisiae Mitochondria;136
6.6;Chapter 6. LacZ Assays in Yeast;151
6.7;Chapter 7. Analysis of Budding Patterns;170
6.8;Chapter 8. Uses and Abuses of HO Endonuclease;180
6.9;Chapter 9. Assays for Gene Silencing in Yeast;204
7;Section II: Making Mutants;226
7.1;Chapter 10. Classical Mutagenesis Techniques;228
7.2;Chapter 11. Introduction of Point Mutations into Cloned Genes;238
7.3;Chapter 12. Insertional Mutagenesis: Transposon–Insertion Libraries as Mutagens in Yeast;258
7.4;Chapter 13. Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro;269
7.5;Chapter 14. Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae;287
7.6;Chapter 15. Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Mediated PCR;297
7.7;Chapter 16. kar-Mediated Plasmid Transfer between Yeast Strains: Alternative to Traditional Transformation Methods;317
7.8;Chapter 17. Gene Disruption;329
7.9;Chapter 18. Synthetic Dosage Lethality;355
8;Section III: Genomics;366
8.1;Chapter 19. Saccharomyces Genome Database;368
8.2;Chapter 20. Three Yeast Proteome Databases: PombePD, and CalPD (MycoPathPD);386
8.3;Chapter 21. Database Resources Relevant to Yeast Biology;412
8.4;Chapter 22. Searching Yeast Intron Data at Ares Lab Web Site;419
8.5;Chapter 23. Yeast Genomic Expression Studies Using DNA Microarrays;432
8.6;Chapter 24. Transcriptome Analysis of Saccharomyces cerevisiae Using Serial Analysis of Gene Expression;453
8.7;Chapter 25. PCR-Based Engineering of Yeast Genome;484
8.8;Chapter 26. ChIP-chip: A Genomic Approach for Identifying Transcription Factor Binding Sites;508
8.9;Chapter 27. Computational Approaches to Identifying Transcription Factor Binding Sites in Yeast Genome;523
9;Section IV: Proteomics;536
9.1;Chapter 28. Array-Based Methods for Identifying Protein Protein and Protein–Nucleic Acid Interactions;538
9.2;Chapter 29. Building Protein–Protein Networks by Two- Hybrid Mating Strategy;552
9.3;Chapter 30. Integrated Version of Reverse Two-Hybrid System for the Postproteomic Era;564
9.4;Chapter 31. Biochemical Genomics Approach to Map Activities to Genes;585
9.5;Chapter 32. Use of Two-Dimensional Gels in Yeast Proteomics;598
10;Author Index;624
11;Subject Index;648