Guo / Haque RNA Nanotechnology and Therapeutics

Methods and Protocols
2015
ISBN: 978-1-4939-2562-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1297, 239 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume contains a compilation of techniques and laboratory protocols directly related to RNA nano technology and its applications in nano biotechnology and nano medicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nano architectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nano constructs
in vivo;
conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful
Methods of Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.

Cutting-edge and practical,
RNA Nanotechnology and Therapeutics: Methods and Protocols
propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Guo, Peixuan

Haque, Farzin

Inhaltsverzeichnis

Overview of Methods in RNA Nanotechnology - Synthesis, Purification and Characterization of RNA Nanoparticles.- Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-Assembly.- Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM.- Silver Nano clusters for RNA Nano technology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies.- Purification of RNA Nanoparticles by Ultracentrifugation.- HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single Nucleotide Resolution.- RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover
In Vivo.-
Fluorescence Labeling of Short RNA by Oxidation at the 3’-End.- Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases.- Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor.- Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide-Protein Complexes to SELEX Screens.- Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge.- A High Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells.- Design, Assembly, and Evaluation of RNA-Protein Nanostructures.- Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq.- Mapping Protein-RNA Interactions by RCAP, RNA-Crosslinking and Peptide Fingerprinting.

Über Autor(innen)

Peixuan Guo, Ph. D. William Farish Endowed Chair in Nano biotechnology Professor of School of Pharmacy Director of Nano biotechnology Center University of Kentucky Director of NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy Mailing address College of Pharmacy University of Kentucky 565 Biopharm Complex 789 S. Limestone Street Lexington, KY 40536 Phone: (Office) [phone] (Cell Phone): [phone] [e-mail] (future)