Guest Pre-Clinical Models

Techniques and Protocols
Erscheinungsjahr 2018
ISBN: 978-1-4939-8994-2
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1916, 343 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pre-Clinical Models: Techniques and Protocols aims to provide methods that describe the context of specific disease or therapeutic areas.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Guest, Paul C.

Inhaltsverzeichnis

A Guide to Mass Spectrometry-based Quantitative Proteomics.- Mitochondrial Involvement in Mental Disorders; Energy Metabolism, Genetic and Environmental Factors.- Monosodium Glutamate (MSG)-induced Animal Model of Type 2 Diabetes.- The Elevated Plus Maze Test for Measuring Anxiety-like Behaviour in Rodents.- The Forced Swim Test for Depression-like Behaviour in Rodents.- Object Burying Test for Assessment of Obsessive Compulsive Behaviours in Mice.- The Nest Building Test in Mice for Assessment of General Well-being.- Mouse Free Dyadic Social Interaction Test.- The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behaviour.- The Y-maze for Assessment of Spatial Working and Reference Memory in Mice.- Maturation of a Human Oligodendrocyte Cell Line.- Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan.- Isolation and Characterisation of Different Mesenchymal Stem Cell Populations from Rat Femur.- Characterization of an Animal Model of Autism and Social Interaction.- Assaying Reproductive Capacity in Female Rodents.- Experimental Model of Hindlimb Suspension-Induced Skeletal Muscle Atrophy in Rodents.- An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes using Flow Cytometry.- Characterization of the db/db Mouse Model of Type 2 Diabetes.- Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes.- Characterization of Human Transplantable Insulinoma Cells.- Preparation of Islets from Rat Pancreas and Assessment of Islet Function.- Cryopreservation of Red Blood Cells.- Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryo-preservation.- Reprogramming of Human Melanocytes and Melanoma cells with Yamanaka Factors.- The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis.- Generation of Human Pyruvate Carboxylase Knockout Cell Lines UsingRetrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study its Metabolic Role in Cancer Research.- The use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity.- OVA-induced Allergic Airway Inflammation Mouse Model.- Description of Ovariectomy Protocol in Mice.- A Rat Eye Lens Model of Cataract Formation.- A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue.- Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 ß-Cell Line– a Preclinical Model for Type 2 Diabetes.