DNA Sequencing Protocols

2. Auflage 2001
ISBN: 978-0-89603-716-8
Verlag: Humana Press

Buch, Englisch, Band 167, 244 Seiten, Book, Format (B × H): 152 mm x 229 mm, Gewicht: 1 g

Reihe: Methods in Molecular Biology

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

Zielgruppe

Research

Autoren/Hrsg.

Graham, Colin A

Hill, Alison J.M.

Inhaltsverzeichnis

DNA SEQUENCING PROTOCOLS, Second Edition
Edited by Colin A. Graham and Alison J. M. Hill

Contents

1. Introduction to DNA sequencing
Colin A. Graham and Alison J. M. Hill
2. M13 cloning
Joachim Messing
3. M13 sequencing
Qingzhong Yu
4. Primer Design and Primer directed sequencing
Ulrike Gerischer and Peter Dürre
5. Direct sequencing of DNA produced in a Polymerase Chain Reactio
Ulrike Gerischer
6. Solid phase fluorescent sequencing of the CFTR gene
Harry Cuppens and Jean-Jacques Cassiman
7. Shotgun sequencing
Alan T. Bankier
8. Cycle sequencing
Robert W. Blakesley
9. Cycle Sequencing of PCR-Amplified Genomic DNA with Dye-Labelled Universal Primers
Johannes Zschocke and Georg F. Hoffmann
10. Automated fluorescent DNA sequencing on the ABI PRISM 377
Jonathan R. E. MacBeath, Suzanna S. Harvey, and Nicola J. Oldroyd
11. Automated fluorescent DNA sequencing on the ABI PRISM 310 genetic analyser
David Watts and Jonathan R. E. MacBeath
12. Fluorescent sequencing protocols for the ALF
Shirley MacQuaid
13. Fluorescent sequencing for heterozygote mutation detection
Colin A. Graham and Alison J. M. Hill
14. Automation in DNA sequencing
Alan T. Bankier
15. Sequence databases and the internet
Anne E. Hughes

Introduction to DNA Sequencing. M13 Cloning. M13 Sequencing. Primer Design and Primer Directed Sequencing. Direct Sequencing of DNA Produced in a Polymerase Chain Reaction. Solid Phase Fluorescent Sequencing of the CFTR Gene
Shotgun Sequencing. Cycle Sequencing. Cycle Sequencing of PCR-Amplified Genomic DNA With Dye-Labelled Universal Primers. Automated Fluorescent DNA Sequencing on the ABI PRISM 377. Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyser. Fluorescent Sequencing Protocols for the ALF
Fluorescent Sequencing for Heterozygote MutationDetection. Automation in DNA Sequencing. Sequence Databases and the Internet.