García-Ortega / Glieder / Kovar Pichia pastoris
Erscheinungsjahr 2025
ISBN: 978-1-0716-4779-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
Methods and Protocols
E-Book, Englisch, 682 Seiten
Reihe: Springer Protocols
ISBN: 978-1-0716-4779-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark
This volume provides readers with a useful and comprehensive resource of laboratory protocols for working with
. The chapters in this book are organized into seven parts: introduction, bioinformatics, cell and systems biology, gene expression cassettes and vectors,
expression strains, upstream bioprocessing, and downstream processing and product analysis. This book covers basic and newer laboratory methods, starting from the gene and genetic construct to mature (yet reasonable) amounts of a recombinant product being produced, purified, and characterized. Written in the highly successful
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough,
is a valuable resource for both new and experienced researchers who want to learn more about this important and developing field.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
A Brief History of the
Expression System.-
Genomic Resources: Accessibility, Recommendations, and Pitfalls.- Isolation of
Genomic DNA for Long-Read DNA Sequencing and PCR Applications.-
RNA-Sequencing and Data Analysis.- Localization of Proteins in the Secretory Pathway of
using Fluorescence Microscopy.- Auxin-Induced Degradation of Target Proteins in
Establishing Reference Genes of
Quantitative Real-Time Polymerase Chain Reaction Analyses.- ID-MS Based Quantitative Analysis of Metabolites in
A Step-By-Step Protocol.- High Throughput Stationary
C-Based Metabolic Flux Analysis of
Strains.- Determination of Biomass Elemental Composition of
Expression Plasmid Assembly using Type IIS Restriction Enzymes.- Autonomously Replicating Sequence-Bearing Plasmids Utilized in
Overlap Extension Construction and Direct Cloning of Loop-Out Prone DNA Expression Cassettes in
75k-?-Secalin from
(rye) as a Case Study.- In Vivo Plasmid Assembly in NHEJ-Deficient
Strains.- Protein Targeting Signals and Strategies for
Cloning of MFa Secretion Variants for Protein Secretion in
Lytic Polysccharide Monooxygenases as Reporters for Signal Peptide Evaluation in
Exploting Self-Processing 2A Peptides for Multi-Gene Expression in
Retrofitting of
Promoter-Based
Expression Strains for Methanol-Independent Protein Production.- Selective Cultivation of Methylotrophic
Laboratory and Natural Strains.- Transformation of
by Electroportation.- High Throughput Transformation of Chemical Competent
Cells using Lithium Acetate/Chloride and Carrier DNA.- High-Efficiency Transformation of Integrative Libraries under Zeocin Selection in
High-Throughput Generation of
Knock-Out Strains by using CRISPR/Cas9.- Engineering
Strains using CRISPR/Cas9 Technologies: The Basic Protocol.- Adaptive Laboratory of
by Serial Batch Cultivation.- Classical Genetics of
Random Mutagenesis and Screening.- Cryo-Preservation of
Strains for Long-Term Storage.- Adapting
Cultivation Media to REACH Regulations.- Fed-Batch Operational Strategies for Efficient and Robust
Cultivations Achieving High Cell Densities.- A Guide to Fed-Batch Cultivation of
on Laboratory Scale.-
Continuous Cultivations for Strain Characterization and Continuous Biomanufacturing.- Best Practices for Protein Expression in
Combining Design of Experiments with an Automated Platform for Parallelized Cultivation in Small-Scale Stirred Tank Bioreactors.- Kinetic Modeling of
Bioreactor Systems under Different Operating Conditions: Batch, Fed-Batch, and Chemostat.- On-Line Monitoring and Control of Methanol Concentration in
Fermentations.- Off-Gas Balancing for Bioprocess Monitoring
Bioprocesses.- A Single-Use Technology Platform: How to Set Up Bioprocesses with
A
Cell-Free Protein Synthesis Platform.- Clarification of
Broth by TFF with Ceramic Membranes.-
Cell Disruption by High-Pressure Homogenization (HPH).- Concentration and Purification of Proteins Produced by
by TFF with Ceramic Membranes.- Protein Purification Protocols for Recombinant Enzymes Produced in
Characterization of Low Molecular Weight Variants (Fragmentation) in
by CE-SDS.
