Fukuda Glycomics

1. Auflage 2006
ISBN: 978-0-08-054945-3
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 416, 456 Seiten, Web PDF

Reihe: Methods in Enzymology

In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.

Autoren/Hrsg.

Fukuda, Minoru

Inhaltsverzeichnis

1;Cover;1
2;Copyright Page;5
3;Table of Contents;6
4;Contributors to Volume 416;10
5;Preface;14
6;Volume in Series;16
7;Section I: Carbohydrate–Protein Interactions–Physical Measurements;40
7.1;Chapter 1. Characterization of Specific Donor Binding to a1,4-N-Acetylhexosaminyltransferase EXTL2 Using Isothermal Titration Calorimetry;42
7.2;Chapter 2. NMR Analysis of Carbohydrate–Protein Interactions;51
7.3;Chapter 3. X-ray Crystal Structure Determination of Mammalian Glycosyltransferases;69
8;Section II: Phage Display Library;88
8.1;Chapter 4. Screening of Peptide-Displaying Phage Libraries to Identify Short Peptides Mimicking Carbohydrates;90
8.2;Chapter 5. Phage Display-Derived Human Antibodies Against Specific Glycosaminoglycan Epitopes;100
9;Section III: Glycosyltransferase Expression Profiling;128
9.1;Chapter 6. Comprehensive Enzymatic Characterization of Glycosyltransferases with a ß3GT or ß4GT Motif;130
9.2;Chapter 7. Autofluorescent Proteins for Monitoring the Intracellular Distribution of Glycosyltransferases;141
9.3;Chapter 8. Expression Profiling of Glycosyltransferases and Related Enzymes Using In Situ Hybridization;159
9.4;Chapter 9. Expression Profiling of Glycosyltransferases Using RT-PCR;168
9.5;Chapter 10. Expression Profiling of Glycosyltransferases and Related Enzymes Using Gene Microarrays;180
10;Section IV: CHO Mutants in Glycan Biosynthesis;196
10.1;Chapter 11. Lectin-Resistant CHO Glycosylation Mutants;198
10.2;Chapter 12. CHO Glycosylation Mutants: GPI Anchor;221
10.3;Chapter 13. CHO Glycosylation Mutants: Proteoglycans;244
11;Section V: Function of Sulfation;262
11.1;Chapter 14. Determination of Substrate Specificity of Sulfotransferases and Glycosyltransferases (Proteoglycans);264
11.2;Chapter 15. Measuring the Activities of the Sulfs: Two Novel Heparin/Heparan Sulfate Endosulfatases;282
11.3;Chapter 16. Determination of Chemokine–Glycosaminoglycan Interaction Specificity;293
11.4;Chapter 17. Identification of Proteoglycan-Binding Proteins;302
11.5;Chapter 18. Functions of Glycans Revealed by Gene Inactivation of L-Selectin Ligand Sulfotransferases in Mice;318
12;Section VI: Functions Revealed by Forced Expression or Gene Knockout;330
12.1;Chapter 19. Expression of Specific Carbohydrates by Transfection with Carbohydrate Modifying Enzymes;332
12.2;Chapter 20. N-Glycan Structure Analysis Using Lectins and an a-Mannosidase Activity Assay;343
12.3;Chapter 21. Targeted Disruption of the Gene Encoding Core 1ß1-3-Galactosyltransferase (T-Synthase) Causes Embryonic Lethality and Defective Angiogenesis in Mice;353
12.4;Chapter 22. Roles of Mucin-Type O-Glycans Synthesized by Core2ß1,6-N-Acetylglucosaminyltransferase;371
12.5;Chapter 23. Analysis of Leukocyte Rolling In Vivo and In Vitro;385
12.6;Chapter 24. Cell Type–Specific Roles of Carbohydrates in Tumor Metastasis;410
13;Author Index;420
14;Subject Index;446
15;Color Plate Section;454