Fedoroff / Richardson Protocols for Neural Cell Culture

3rd Auflage 2001
ISBN: 978-1-59259-207-4
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 262 Seiten, eBook

Reihe: Springer Protocols Handbooks

The first edition of Protocols for Neural Cell Culture was published in 1992 and the second edition in 1997. Originally, the publication grew outofprotocols used in the Tissue Culture Course given at the University of Saskatchewan. The course was patterned on those given by the Tissue CultureAssociation, first in Toronto, Canada, in 1948, then in Cooperstown, NY, then Denver, CO, and finally in Madison, WI, where the course ended in 1964. The course in Saskatchewan began in 1963 as a month-long international course that included both animal and plant tissue cultures. Over the years the course underwent specialization, first being limited to animal tissue culture, then to an intensive one-week general course. This led to one-week courses especially designed for tissue culture for the study of cancer or of the cardiovascular or the nervous system. In 1989, the Saskatchewan course became part of the Tissue Culture Training Facility of the Neuroscience Network of the Canadian Network of Centres of Excellence. The course and the Training Facility ceased to exist in 1997. The faculty for the Saskatchewan course was drawn from the best labora tories in the world and laboratory protocols from those centers were thoroughly tested in a student laboratory setting for many years.

Zielgruppe

Research

Autoren/Hrsg.

Fedoroff, Sergey

Richardson, Arleen

Inhaltsverzeichnis

In Vitro Assays for Axonal Growth and Targeting.- Interface Organotypic Hippocampal Slice Cultures.- Slice Cultures for Study of Microglia.- Microexplant Cultures of the Cerebellum.- Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons.- Chick Spinal Somatic Motoneurons in Culture.- Primary Cultures of Sympathetic Ganglia.- Primary Cell Cultures for the Study of Myelination.- Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures.- Culture of Glial Cells from Human Brain Biopsies.- Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium.- Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium.- Olfactory Ensheathing Cell Cultures.- Cultures of Stem Cells of the Central Nervous System.- Aggregating Neural Cell Cultures.- Neural Cell Lines.- Virus Vectors for Gene Therapy of the Nervous System.- Preparation of Substrata for In Vitro Culture of Neurons.- Serum-Free Media forNeural Cell Cultures.- Development of Multiple-Well Plate Biological Assays.- Neural Cell Counting.- Assay for Neuronal Cell Migration.- Colony Cultures.- Hybridoma Cultures for Production of Antibodies.- Elimination of Cell Types from Mixed Neural Cell Cultures.- Quantification of Cells in Culture.- Tips for Tissue Culture.