Fedoroff / Richardson Protocols for Neural Cell Culture

2. Auflage 1997
ISBN: 978-1-4757-2586-5
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 278 Seiten, eBook

This volume is designed to be kept close at hand as a ready reference and a guide to laboratory procedures. It is based on tissue culture manuals used for a number of years at international courses on tissue culture at the University of Saskatchewan, made possible by the generous support of the Canadian Council of Animal Care and the Medical Research Council of Canada. Sergey Fedoroff Arleen Richardson The second edition of Protocols for Neural Cell Culture adheres to the prin ciples enunciated in the first edition, but the content has been extensively revised and expanded. Two new chapters have been added to reflect the increased interest in the development and differentiation of the nervous system and in the reconstruction of its circuitry in tissue culture. One chapter deals with slice cul tures in which the organization of the nervous system is preserved. When slice cultures are combined with explant cultures, afferent and efferent projections can be reconstructed. The other chapter deals with aggregating neural cell cul tures, in which "minibrains" can form. Theses are small, uniformly sized spheres of nervous tissue, usually having nerve cells in the center and astrocytes, oligo dendrocytes, and microglia in the periphery. Such cultures can be used to study neutral cell interactions in an organized milieu and for qualitative as well as quantitative studies at biochemical and molecular levels.

Zielgruppe

Research

Autoren/Hrsg.

Fedoroff, Sergey

Richardson, Arleen

Inhaltsverzeichnis

One Outgrowth Assays and Cortical Slice Cultures.- Two Microexplant Cultures of the Cerebellum.- Three Aggregating Neural Cell Cultures.- Four Primary Cell Cultures of Peripheral and Central Neurons and Glia.- Five Chick Spinal Somatic Motoneurons in Culture.- Six Neural Cells in Microcultures: In Vitro Biological Assays for Neuroactive Agents.- Seven Construction and Use of Compartmented Cultures.- Eight Astrocyte and Oligodendrocyte Cultures.- Nine Generation of Mouse Astroglia and Microglia Cultures from Mouse Neopallium.- Ten Generation of Oligodendroblasts from Primary Cultures of Rat Neopallium.- Eleven Culture of Glial Cells from Human Brain Biopsies.- Twelve Colony Cultures.- Thirteen Herpes Simplex Virus Vectors for Gene Therapy of the Nervous System.- Fourteen Immunostaining and Identification of Antigens.- Fifteen Elimination of Cell Types from Mixed Neural Cell Cultures.- Sixteen Quantification of Cells in Culture.- Seventeen Procedures for Subculturing Cells.- Eighteen Preparation of Tissue-Cultured Material for Electron Microscopical Observation.- Nineteen Tips in Tissue Culture.- Twenty Use of Dissecting Instruments in Tissue Culture.- Abbreviations and Definitions.