Espina / Liotta Molecular Profiling

Methods and Protocols
2012
ISBN: 978-1-60327-216-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 823, 447 Seiten, eBook

Reihe: Methods in Molecular Biology

The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy. Molecular profiling technology has advanced dramatically, particularly in the field of cancer tissue biomarkers. It is now possible to gather complex genomic and proteomic information from a routine clinical needle biopsy or surgical specimen. In Molecular Profiling : Methods and Protocols, expert researchers in the field focus on the entire process from discovery to commercialization, with practical guides that are not limited to experimental methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as essays and guidelines for grants, patents, and commercialization of products related to molecular profiling.

 Authoritative and practical, Molecular Profiling: Methods and Protocols seeks to aid scientists in understanding the latest advancements in genomics, proteomics, imagining, and bioinformatics.

Zielgruppe

Research

Autoren/Hrsg.

Espina, Virginia

Liotta, Lance A.

Inhaltsverzeichnis

ContentsPrefaceContributors1. Tumor Staging and Grading: A Primer Stacy M. Cowherd2. Clinical Trial Design in the Age of Molecular Profiling Alexander Spira and Kirsten H. Edmiston3. Personalized Medicine: Ethics for Clinical Trials G. Terry Sharrer4. Reduction of Preanalytical Variability in Specimen Procurement for Molecular Profiling Virginia Espina and Claudius Mueller5. The Human Side of Cancer Biobanking Eoin Gaffney, Deirdre Madden, and Geraldine A. Thomas6. Introduction to Genomics Luca Del Giacco and Cristina Cattaneo7. Genomic Profiling: cDNA arrays and oligoarrays Francesco Gorreta, Walter Carbone, Dagania Barzhagi8. Genome wide methylation profiling in archival formalin fixed paraffin embedded (FFPE) tissue samplesJ. Keith Killian, Robert L. Walker, Sven Bilke, Yidong Chen, Sean Davis, Robert Cornelison, William I. Smith, and Paul S. Meltzer9. An Overview of microRNA Methods: Expression Profiling and Target Identification Sinéad M Smith and David W Murray10. Antibody Validation by Western Blotting Michele Signore and K. Alex Reeder11. Laser Capture Microdissection: ArcturusXT infra-red capture and UV cutting methods Rosa I. Gallagher, Steven R. Blakely, Lance A. Liotta, and Virginia Espina12. Antibody microarrays: Analysis of Cystic FibrosisCatherine E. Jozwik, Harvey B. Pollard, Meera Srivastava, Ofer Eidelman, QingYuan Fan, Thomas N. Darling, and Pamela L. Zeitlin13. Tissue Microarrays As A Tool In The Discovery & Validation Of Predictive Biomarkers Stephen M. Hewitt14. Reverse phase protein microarrays Mariaelena Pierobon, Francesca Galdi, Noemi Moroni, and Emanuel F. Petricoin III15. Serum Low Molecular Weight Protein Fractionation for Biomarker DiscoveryAmy VanMeter, Serena Camerini, Maria Letizia Polci, Alessandra Tessitore, Nishant Trivedi, Michael Heiby, Jonathan Hansen, and Weidong Zhou16. Mass spectrometry based biomarker discovery Weidong Zhou, Emanuel F. Petricoin III, and Caterina Longo17. Mitochondrial Proteome: Toward The Detection and Profiling of Disease Associated Alterations Paul C. Herrmann and E. Clifford Herrmann18. Adult Neural Stem Cells: Isolation and Propagation Jamin M. Lechter and Daniel N. Cox19. Evanescent Wave Imaging: An Introduction to Total Internal Reflection Fluorescence Microscopy (TIRF) Bryan A. Millis20. Construction and Hyperspectral Imaging of Quantum Dot Lysate Arrays Kevin P. Rosenblatt, Michael Huebschman, and Harold R. Garner21. Microarray Data Analysis: Comparing Two Population Means Jianghong Deng, Valerie S. Calvert and Mariaelena Pierobon22. Bioinformatics/Biostatistics: microarray analysis Gabriel S. Eichler23. Structure-based functional design of drugs: from target to lead compound Amy C. Anderson24. Personalized Medicine: Changing the Paradigm of Drug Development Robin Couch and Bryan T. Mott25. Grant Writing Tips for the First Time Applicant Lindsay Wescott and Michael Laskofski26. Inventions and Patents: A practical tutorial J. Lille Tidwell and Lance Liotta27. Regulatory Approval Pathways for Molecular Diagnostic Technology Lance Liotta and Emanuel Petricoin28. Small business development for molecular diagnostics Athanasia Anagnostou and Lance Liotta