Enzyme Assays

2. Auflage 2002
ISBN: 978-0-19-963820-8
Verlag: Oxford University Press

Buch, Englisch, 302 Seiten, Print PDF, Format (B × H): 191 mm x 247 mm, Gewicht: 594 g

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.

Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration.

This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Autoren/Hrsg.

Eisenthal, Robert

Danson, Michael J.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Biochemie (med.)
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Physiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Naturwissenschaften Biowissenschaften Enzymologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Naturwissenschaften Chemie Analytische Chemie
• Naturwissenschaften Biowissenschaften Lipidologie

Inhaltsverzeichnis

- 1: Keith F Tipton: Principles of enzyme assay and kinetic studies

- 2: Robert A John: Photometric assays

- 3: Kelvin T Hughes: Radiometric assays

- 4: Shabih E H Syed: High performance liquid chromatography assays

- 5: J B Clark: Electrochemical assays: the oxygen electrode

- 6: R D Hurst and J B Clark: Electrochemical assays: the nitric oxide electrode

- 7: Keith Brocklehurst: Electrochemical assays: the pH-stat

- 8: Gunter M Rothe: Enzyme assays after gel electrophoresis

- 9: Nicholas C Price and Lewis Stevens: Techniques for enzyme extraction

- 10: Mark T Martin: Determination of active site concentration

- 11: David Hayes and Geoff Mellor: High throughput screening - considerations for enzyme assays

- 12: Athel Cornish-Bowden: Statistical analysis of enzyme kinetic data