Durack / Robinson Emerging Tools for Single-Cell Analysis

Advances in Optical Measurement Technologies
1. Auflage 2004
ISBN: 978-0-471-46100-5
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 360 Seiten, E-Book

Reihe: Cytometric Cellular Analysis

The resurgence of interest in high-resolution evaluation ofsingle-cell properties has led to examining where currenttechnology stands at the beginning of a new millennium. Engineersand scientists have produced significant advances in cytometrictechnologies in just the past few years. Emerging Tools forSingle-Cell Analysis: Advances in Optical MeasurementTechnologies stresses the applications and theories behindsome of these advances in cell measurement and cell- sortingtechnologies.
Rapid assessment of the proper function of cells and molecularprocesses within cells is essential. To that end, new and varyingtechnologies present important diagnostic and prognostic toolsrelevant to a variety of diseases. Future developments inminiaturization of electronics, micro- and nanomachines, andbiomedical engineering are certain to impact cell biology. Newanalytical technologies are revolutionizing our ability tofunctionally characterize, isolate, and manipulate single cells.This timely book offers researchers and design engineersmuch-needed information as they further develop technologies forcell analysis.
By comparing and contrasting various approaches, the authorsexplain how those technologies converge toward similar goals:evaluating the properties of cells and sorting cells on thoseproperties using optically-based measurement systems.Emerging Tools for Single-Cell Analysis offersscientists and engineers a vision of the exciting possibilitiesthat exist as new technologies are applied to single-cellanalysis,

Autoren/Hrsg.

Durack, Gary

Robinson, J. Paul

Inhaltsverzeichnis

Cell-Sorting Technology (G. Durack).
High-Speed Cell Sorting (G. van den Engh).
Rare-Event Detection and Sorting of Rare Cells (J. Leary).
Applications of High-Speed Sorting of CD34 + HematopoieticStem Cells (T. Leemhuis & D. Adams).
Microfabricated Fluidic Devices for Single-Cell Handling andAnalysis (D. Beebe).
Single DNA Fragment Detection by Flow Cytometry (R. Habbersett, etal.).
Fluorescence Lifetime Imaging: New Microscopy Technologies (W. Yu,et al.).
Fluorescence Lifetime Flow Cytometry (J. Steinkamp).
Application of Fluorescence Lifetime and Two-Photon FluorescenceCytometry (D. Weaver, et al.).
Probing Deep-Tissue Structures by Two-Photon FluorescenceMicroscopy (C.-Y. Dong, et al.).
Limits of Confocal Imaging (J. Pawley).
Scanning Near-Field Optical Imaging and Spectroscopy in CellBiology (V. Subramaniam, et al.).
White-Light Scanning Digital Microscopy (J. Robinson & B.Gravely).
Illumination Sources (H. Shapiro).
Camera Technologies for Cytometry Applications (K.Castleman).
Index.