Buch, Englisch, Band 2218, 383 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 947 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 2218, 383 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 947 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-0716-0969-9
Verlag: Springer US
This volume details a wide range of methods, ranging from beginner through advanced, used to
further study zebrafish and fish germline.. Chapter guide readers through cultivating and manipulating germ cells, imaging of germline processes and the molecular analysis of their, protein, and RNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Germline Development in the Zebrafish: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Zielgruppe
Professional/practitioner
Autoren/Hrsg.
Weitere Infos & Material
1. Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish
Nana Ai, Lin Liu, Esther Shuk-Wa Lau, Anna Chung-Kwan Tse, and Wei Ge
2. The Xenopus Oocyte as an Expression System for Functional Analyses of Fish Aquaporins
François Chauvigné, Alba Ferré, and Joan Cerdà
3. Computer-Assisted Sperm Analysis to Test Environmental ToxicantsTímea Kollár, Ákos Horváth , and Zsolt Csenki-Bakos
4. Cryopreservation and Transplantation of Spermatogonial Stem Cells
Zoran Marinovic, Jelena Lujic, Qian Li, Yoshiko Iwasaki, Béla Urbányi, Goro Yoshizaki, and Ákos Horváth
5. Masculinization of Zebrafish through Partial Depletion of Primordial Germ Cells by Injecting Diluted Morpholino Oligonucleotides into Embryos
László Orbán, Jolly M. Saju, Keh-Weei Tzung,and Woei Chang Liew
6. Chemical Genetics: Manipulating the Germline with Small MoleculesYoungnam N. Jin and Randall T. Peterson
7. In vitro Induction of Teleost PGCs
Vanesa Robles, David G. Valcarce,and Marta F. Riesco
8. Applying Rho Pathway Inhibitors to Investigate Germ Plasm LocalizationJerónimo Mirandaand Denhí Schnabel
9. Cryopreservation of Pooled Sperm Samples
Jennifer L. Matthews and Zoltan M. Varga
10. Quantifying Tissue Tension in the Granulosa Layer after Laser Surgery
Peng Xiaand Carl-Philipp Heisenberg
11. In-vivo and Ex-vivo CT Imaging of Zebrafish Gonads
Christian Dullinand Louisa Habich
12. Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries
Avishag Mytlis and Yaniv M. Elkouby
13. Detection of the Polar Body after Fertilization
Hua Ruan, Xiaogui Yi and Honghui Huang
14. Labelling the Micropylar Cell in Zebrafish Whole-mount and Cryo-sectioned Follicles
Chaitanya Dingare, Petra A. Klemmt, Alina Niedzwetzki,Virginie Lecaudey
15. Visualisation of Transcriptional Activity in Early Zebrafish Primordial Germ CellsFabio M. D’Orazio, Aleksandra Jasiulewicz, Yavor Hadzhiev, and Ferenc Müller
16. Experimental Methodologies to Visualise Early Cell Biology in Zebrafish Embryos
Asfa Sabrin Borbor and Sreelaja Nair17. Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy
Toshiya Nishimura and Minoru Tanaka
18. Methods for Visualization of RNA and Cytoskeletal Elements In The Early Zebrafish Embryo
Hansen, C.L. and Pelegri, F.
19. Glyoxal Fixation as an Alternative for Zebrafish Embryo Immunostaining
Nadia Rostamand Roland Dosch
20. Histological Analysis of Gonads in Zebrafish
Kellee R. Siegfried and Jocelyn S. Steinfeld
21. Manipulating and Visualizing the Germline with Transgenic Lines
Ding Yeand Yonghua Sun
22. Liquid Chromatography and Tandem Mass Spectrometry in Label Free Protein Quantification of Zebrafish (Danio rerio) Eggs
Ozlem Yilmaz, Emmanuelle Com, Regis Lavigne, Charles Pineau, and Julien Bobe
23. In vivo Protein Lifetime Measurements across Multiple Organs in the Zebrafish
Sunit Mandad, Gudrun Kracht, and Eugenio F. Fornasiero
24. In vivo Imaging of Protein Interactions in the Germ Plasm with Bimolecular Fluorescent Complementation
Roshan Perera and Roland Dosch25. The Zygotic Genome Activation: Critical Prelude to the Most Important Time of Your Life
Vladimir Korzh
26. Identification of RNA-binding Protein Landscapes Across Zebrafish Embryonic Transcriptome via iCLIP Approach
Vladimir Despic
27. Tethered Function Assay to Study RNA Regulatory Proteins in Zebrafish Embryos
Yuichiro Mishima and Kunio Inoue
28. Massively Parallel Analysis of Regulatory RNA Sequences
Michal Rabani
29. Exploring Translational Control of Maternal mRNAs in Zebrafish
Cecilia Lanny Winata, Maciej Lapinski, Hisyam Ismail, Sinnakaruppan Mathavan, and Prabha Sampath
